PDF(Pubmed)
Abstract：
Bats are a significant reservoir for numerous pathogens, including Bartonella spp. It is one of the emerging zoonotic bacterial diseases that can be transmitted to humans and may cause various unspecific clinical manifestations. Thus, bartonellosis is rarely diagnosed and is regarded as a neglected vector-borne disease (VBD). Bat flies have been hypothesised to be a vector in the transmission of pathogens among bats. They are host-specific, which reduces the likelihood of pathogen transmission across bat species; however, they are likely to maintain high pathogen loads within their host species. To explore the presence of Bartonella spp. in bat flies from Peninsular Malaysia; bat fly samples collected from various sites at the east coast states were subjected to molecular detection for Bartonella spp. It was discovered that 38.7 % of bats from Terengganu and Kelantan were infested with bat flies; however, no bat fly was found in bats collected from Pahang. The collected bat flies belonged to the families Nycteribiidae (79.6 %) and Streblidae (20.4 %). The collected bat flies were pooled according to the locations and species into 39 pools. Out of these 39 pools, 66.7 % (n = 26) were positive for Bartonella spp. by PCR. Sequence analyses of five randomly selected PCR-positive pools revealed that pools from Kelantan (n = 3) have the closest sequence identities (99 %) to Bartonella spp. strain Lisso-Nig-922 from Nigeria. However, the other pools from Terengganu (n = 2) were closely related to Bartonella spp. strain KP277 from Thailand and Bartonella spp. strain Rhin-3 from the Republic of Georgia with 99 % and 100 % sequence identity, respectively. This suggests that the Bartonella spp. found in Malaysian bat flies are genetically diverse and can potentially serve as reservoirs for pathogenic Bartonella spp.
摘要：
蝙蝠是众多病原体的重要蓄水池，包括巴尔通菌属。它是一种新兴的人畜共患细菌性疾病，可以传播给人类，并可能引起各种非特异性临床表现。因此，巴顿病很少被诊断，被认为是一种被忽视的媒介传播疾病（VBD）。蝙蝠蝇被认为是病原体在蝙蝠之间传播的媒介。它们是特定于主机的，这降低了病原体在蝙蝠物种之间传播的可能性；然而，它们很可能在宿主物种内维持高病原体负荷。探讨巴尔通体的存在。在马来西亚半岛的蝙蝠蝇中；从东海岸各州各个地点收集的蝙蝠蝇样本进行了Bartonellaspp的分子检测。发现登嘉楼和吉兰丹的蝙蝠中有38.7％的蝙蝠被蝙蝠蝇感染；但是，从彭亨收集的蝙蝠中没有发现蝙蝠蝇。收集的蝙蝠蝇属于Nycteribiidae（79.6％）和Streblidae（20.4％）。根据位置和种类将收集的蝙蝠蝇汇集到39个池中。在这39个池中，66.7％（n=26）的巴尔通体阳性。通过PCR。对五个随机选择的PCR阳性池进行的序列分析显示，来自吉兰丹（n=3）的池与Bartonellaspp具有最接近的序列同一性（99％）。来自尼日利亚的Lisso-Nig-922菌株。然而,登嘉楼的其他池（n=2）与巴尔通菌属密切相关。来自泰国和巴尔通菌属的KP277菌株。来自乔治亚共和国的Rhin-3菌株具有99％和100％的序列同一性，分别。这表明巴尔通氏菌属。在马来西亚蝙蝠蝇中发现的基因多样性，有可能成为致病性巴尔通菌的水库。
