■埃塞俄比亚的结核分枝杆菌群(MTBC)的种群结构多种多样,但主要由欧美(谱系4)和东非印度(谱系3)谱系组成。这项研究的目的是描述中部MTBC分离株的遗传多样性,埃塞俄比亚东部和东南部。
■对从阿达玛和哈拉尔结核病参考实验室转诊的患者获得的总共223株MTBC培养分离株进行了分型。收集人口统计学和临床特征。
■六大谱系:欧美(谱系4),东非印第安人(血统3),东亚(谱系2),印度洋(谱系1),鉴定了非洲分枝杆菌(谱系5和谱系6)和埃塞俄比亚(谱系7)。大多数(94.6%)的分离株是欧美和东非印第安人,比例分别为75.3%和19.3%,分别。总的来说,确定了77种不同的spoligotype模式,其中42种已在SITVIT2数据库中注册。其中,27个spoligotypes是独一无二的,而15个簇有2-49个分离株。SIT149/T3_ETH(n=49),SIT53/T1(n=33),SIT21/CAS1_Kili(n=24)和SIT41/Turkey(n=11)是优势型。一种罕见的北京政治模式,在埃塞俄比亚东部也已确定了SIT541。具有已知SIT的子谱系的总体聚类率为71.3%。年龄组(25-34岁)与聚类显着相关。
■我们发现了由T和CAS家族主导的MTBC的异质种群结构,和欧美血统。北京菌株的鉴定,特别是埃塞俄比亚东部罕见的SIT541spoligotype,需要加强监视计划,对这种基因型知之甚少。利用具有优越鉴别力的工具进行大规模调查,比如全基因组测序,有必要深入了解全国MTBC的遗传多样性,这将有助于指导整体控制工作。
UNASSIGNED: The population structure of Mycobacterium tuberculosis complex (MTBC) in Ethiopia is diverse but dominated by Euro-American (Lineage 4) and East-African-Indian (Lineage 3) lineages. The objective of this study was to describe the genetic diversity of MTBC isolates in Central, Eastern and Southeastern Ethiopia.
UNASSIGNED: A total of 223 MTBC culture isolates obtained from patients referred to Adama and Harar TB reference laboratories were spoligotyped. Demographic and clinical characteristics were collected.
UNASSIGNED: Six major lineages: Euro-American (Lineage 4), East-African-Indian (Lineage 3), East Asian (Lineage 2), Indo-Oceanic (Lineage 1), Mycobacterium africanum (Lineage 5 and Lineage 6) and Ethiopian (Lineage 7) were identified. The majority (94.6 %) of the isolates were Euro-American and East-African-Indian, with proportions of 75.3 % and 19.3 %, respectively. Overall, 77 different spoligotype patterns were identified of which 42 were registered in the SITVIT2 database. Of these, 27 spoligotypes were unique, while 15 were clustered with 2-49 isolates. SIT149/T3_ETH (n = 49), SIT53/T1 (n = 33), SIT21/CAS1_Kili (n = 24) and SIT41/Turkey (n = 11) were the dominant spoligotypes. A rare Beijing spoligotype pattern, SIT541, has also been identified in Eastern Ethiopia. The overall clustering rate of sub-lineages with known SIT was 71.3 %. Age group (25-34) was significantly associated with clustering.
UNASSIGNED: We found a heterogeneous population structure of MTBC dominated by T and CAS families, and the Euro-American lineage. The identification of the Beijing strain, particularly the rare SIT541 spoligotype in Eastern Ethiopia, warrants a heightened surveillance plan, as little is known about this genotype. A large-scale investigation utilizing a tool with superior discriminatory power, such as whole genome sequencing, is necessary to gain a thorough understanding of the genetic diversity of MTBC in the nation, which would help direct the overall control efforts.