PDF(Pubmed)
Abstract：
This study investigated the presence of Mycobacterium bovis (M. bovis) DNA in archived human sputum samples previously collected from residents who reside adjacent to the M. bovis-endemic Hluhluwe-iMfolozi wildlife park, South Africa (SA). Sixty-eight sputum samples were GeneXpert MTB/RIF Ultra-positive for M. tuberculosis complex (MTBC) DNA but culture negative for M. tuberculosis. Amplification and Sanger sequencing of hsp65 and rpoB genes from DNA extracted from stored heat-inactivated sputum samples confirmed the presence of detectable amounts of MTBC from 20 out of the 68 sputum samples. Region of difference PCR, spoligotyping and gyrB long-read amplicon deep sequencing identified M. bovis (n = 10) and M. tuberculosis (n = 7). Notably, M. bovis spoligotypes SB0130 and SB1474 were identified in 4 samples, with SB0130 previously identified in local cattle and wildlife and SB1474 exclusively in African buffaloes in the adjacent park. M. bovis DNA in sputum, from people living near the park, underscores zoonotic transmission potential in SA. Identification of spoligotypes specifically associated with wildlife only and spoligotypes found in livestock as well as wildlife, highlights the complexity of TB epidemiology at wildlife-livestock-human interfaces. These findings support the need for integrated surveillance and control strategies to curb potential spillover and for the consideration of human M. bovis infection in SA patients with positive Ultra results.
摘要：
这项研究调查了牛分枝杆菌（M.bovis）先前从居住在牛分枝杆菌特有的Hluhluwe-iMfolozi野生动物园附近的居民那里收集的存档人类痰样本中的DNA，南非(南非)。68个痰样品是GeneXpertMTB/RIF结核分枝杆菌（MTBC）DNA超阳性，但结核分枝杆菌培养阴性。从储存的热灭活痰样品中提取的DNA中的hsp65和rpoB基因的扩增和Sanger测序证实了68个痰样品中的20个存在可检测量的MTBC。差异PCR的区域，spoligotyping和gyrB长读扩增子深度测序鉴定了牛分枝杆菌(n=10)和结核分枝杆菌(n=7)。值得注意的是,在4个样本中鉴定了牛分枝杆菌SB0130和SB1474，SB0130先前在当地的牛和野生动物中发现，SB1474仅在邻近公园的非洲水牛中发现。痰中的牛分枝杆菌DNA,住在公园附近的人，强调了SA中的人畜共患传播潜力。鉴定仅与野生动植物特别相关的spoligotypes以及在牲畜和野生动植物中发现的spoligotypes，强调了结核病流行病学在野生动物-牲畜-人类界面的复杂性。这些发现支持需要综合监测和控制策略，以遏制潜在的溢出，并考虑在具有阳性Ultra结果的SA患者中人类牛分枝杆菌感染。
