■生物制造利用改良的微生物系统可持续地生产用于农业的商业上重要的生物分子,能源,食物,材料,和制药行业。然而,需要解决与无损和高通量代谢物筛选相关的技术挑战,以充分释放合成生物学和可持续生物制造的潜力。
■这个观点概述了工业细胞菌株开发计划中使用的当前分析筛选工具,并引入了无标签振动光谱显微镜作为替代对比机制。
■我们概述了当前在设计的“测试”部分中使用的分析仪器,build,test,学习合成生物学的周期。然后,我们重点介绍了拉曼散射和红外吸收成像技术的最新进展,这提高了分子特异性和灵敏度。
■高分辨率化学成像方法的最新发展允许更大的吞吐量,而不影响图像对比度。我们提供了支持与微流体集成以在单细胞水平进行快速筛查所需的未来工作的路线图。
■对代谢物的净表达进行定量,可以鉴定具有导致生物分子产生增加的代谢途径的细胞,这对于提高产量和降低工业生物制造成本至关重要。振动显微镜仪器的技术进步将极大地有利于生物材料作为非破坏性细胞筛选的补充方法。
UNASSIGNED: Biomanufacturing utilizes modified microbial systems to sustainably produce commercially important biomolecules for use in agricultural, energy, food, material, and pharmaceutical industries. However, technological challenges related to non-destructive and high-throughput metabolite screening need to be addressed to fully unlock the potential of synthetic biology and sustainable biomanufacturing.
UNASSIGNED: This perspective outlines current analytical screening tools used in industrial cell strain development programs and introduces label-free vibrational spectro-microscopy as an alternative contrast mechanism.
UNASSIGNED: We provide an overview of the analytical instrumentation currently used in the \"test\" portion of the design, build, test, and learn cycle of synthetic biology. We then highlight recent progress in Raman scattering and infrared absorption imaging techniques, which have enabled improved molecular specificity and sensitivity.
UNASSIGNED: Recent developments in high-resolution chemical imaging methods allow for greater throughput without compromising the image contrast. We provide a roadmap of future work needed to support integration with microfluidics for rapid screening at the single-cell level.
UNASSIGNED: Quantifying the net expression of metabolites allows for the identification of cells with metabolic pathways that result in increased biomolecule production, which is essential for improving the yield and reducing the cost of industrial biomanufacturing. Technological advancements in vibrational microscopy instrumentation will greatly benefit biofoundries as a complementary approach for non-destructive cell screening.