背景:三阴性乳腺癌(TNBC)由于乳腺特异性标志物的频率降低,对组织病理学家提出了诊断挑战。SOX10已成为TNBC的有用诊断标记。我们研究的目的是确定我们队列中SOX-10免疫组织化学(IHC)表达的频率,并评估其与临床病理和组织学特征的相关性。
方法:我们纳入了72例原发性TNBC病例。标本包括切块活检和切除标本。我们用SOX10抗体对这些样本的整个载玻片切片进行染色,并计算其表达频率(%)和H评分。我们应用卡方检验来评估SOX10表达与临床病理和组织学特征如患者年龄之间的相关性。试样类型,肿瘤大小,组织学类型,组织学分级,核多态性,有丝分裂计数,肿瘤浸润淋巴细胞(TIL),坏死,钙化,淋巴管浸润(LVI),淋巴结受累,T级,N阶段。
结果:在42例(58.3%)中观察到SOX10表达,中位H评分为57.5。与切除标本相比(73.5vs41.7%),TIL阴性肿瘤的表达明显高于TIL阳性肿瘤(64.3%vs27.3)。与非化生性肿瘤相比,化生性癌的表达降低(35.7%vs63.8%),但没有达到统计学意义。没有观察到与患者年龄的相关性,肿瘤大小,组织学类型,组织学分级,核多态性,有丝分裂计数,坏死,钙化,LVI,淋巴结受累,T级,N阶段。
结论:SOX10在我们研究的一半以上的TNBC病例中表达,这不仅强调了其诊断效用,而且主张将其与其他乳腺特异性标志物联合应用。该表达与大多数临床病理和组织学特征无关,但与tru-cut活检标本和缺乏TILs的相关性引起了人们对适当固定和宿主免疫的可能作用的关注,分别。
BACKGROUND: Triple-negative breast cancer (TNBC) poses a diagnostic challenge for histopathologists due to the reduced frequency of breast-specific markers.
SOX10 has emerged as a useful diagnostic marker for TNBC. The aim of our study was to determine the frequency of SOX-10 immunohistochemical (IHC) expression in our cohort and assess its correlation with clinicopathological and histological features.
METHODS: We included 72 primary TNBC cases. Specimens included tru-cut biopsies and excision specimens. We stained whole slide sections of these specimens with
SOX10 antibody and calculated its frequency (%) of expression and H-score. We applied the chi-square test to assess the correlation between
SOX10 expression and clinicopathological and histological features such as the patient\'s age, specimen type, tumor size, histological type, histological grade, nuclear pleomorphism, mitotic count, tumor-infiltrating lymphocytes (TILs), necrosis, calcification, lymphovascular invasion (LVI), lymph node involvement, T stage, and N stage.
RESULTS: SOX10 expression was observed in 42 (58.3%) cases with a median H-score of 57.5. The expression was significantly higher in tru-cut biopsy specimens as compared to excision specimens (73.5 vs 41.7%) and TILs negative tumors as compared to TILs positive tumors (64.3% vs 27.3). Metaplastic carcinoma showed reduced expression when compared with non-metaplastic tumors (35.7% vs 63.8%), but statistical significance was not achieved. No correlation was observed with the patient\'s age, tumor size, histological type, histological grade, nuclear pleomorphism, mitotic count, necrosis, calcification, LVI, lymph node involvement, T stage, and N stage.
CONCLUSIONS: SOX10 was expressed in more than half of the TNBC cases of our study which not only highlights its diagnostic utility but advocated its application in combination with other breast-specific markers. The expression didn\'t correlate with the majority of clinicopathological and histological features, but correlation with tru-cut biopsy specimens and absence of TILs draws attention towards possible roles of proper fixation and host immunity, respectively.