龙葵。是一种负责影响反刍动物并导致骨骼和软骨组织改变的植物性钙质植物,在其他人中。据信软骨组织的变化,随着骨骼生长的减少,是由于高降钙素血症,由过量的维生素D引起。然而,我们假设S.glaubhyllumDesf。可以直接作用于软骨细胞,因此,来自新生大鼠长骨骨骨的软骨细胞培养物用作模型,以阐明S.glausuphyllumDesf的直接作用。关于骨骼生长。植物样本是从Cañuelas收集的,阿根廷。植物提取物的等分试样用于定量维生素D(1,25(OH)2D3)。在从32只3天大的Wistar大鼠的长骨骨的骨phy中提取的软骨细胞培养物中测试了三种浓度的植物提取物的作用。对照组(无提取物处理),用不同浓度的植物提取物处理3组:第1组(100μL/L);第2组(1mL/L),和第3组(5mL/L),分别含1×10-9米,1×10-8米,和5×10-8M的1,25(OH)2D3。培养7、14和21天后,MTT测定细胞活力,碱性磷酸酶活性,并进行了用高碘酸希夫(PAS)染色的糖胺聚糖(GAG)的面积百分比的定量。第7天,第3组的所有软骨细胞,即,那些含有最高浓度的植物提取物,死了.在第14和21天,与对照相比,组1和2显示软骨细胞活力的显著降低。在7、14和21天,第1组和第2组的碱性磷酸酶活性明显低于对照组。在第21天,第2组显示具有PAS+GAG的区域显著减少。Sox9、Col2、ColX、和aggrecan。S.glaubhylumDesf。提取物通过降低活力直接影响生长的大鼠软骨细胞,碱性磷酸酶活性,和GAG合成,而不改变Sox9,Col2,ColX,和aggrecan,这可能是植物中毒的动物骨骼生长减少的机制之一。
Solanum glaucophyllum Desf. is a calcinogenic plant responsible for enzootic calcinosis that affects ruminants and causes alterations in bone and cartilaginous tissues, among others. It is believed that changes in cartilage tissue, with reduced bone growth, are due to hypercalcitoninism, caused by excess vitamin D. However, we hypothesized that S. glaucophyllum Desf. can act directly on chondrocytes and therefore, chondrocyte cultures from the epiphysis of the long bones of newborn rats were used as a model to elucidate the direct effects of S. glaucophyllum Desf. on bone growth. Plant samples were collected from Cañuelas, Argentina. An aliquot of the plant extract was used to quantify vitamin D (1,25(OH)2D3). The effects of the three concentrations of the plant extract were tested in cultures of chondrocytes extracted from the epiphyses of the long bones of 32 three-day-old Wistar rats. A control group (without extract), and three groups treated with different concentrations of plant extract were formed: group 1 (100 μL/L); group 2 (1 mL/L), and group 3 (5 mL/L), containing respectively 1 × 10-9 M, 1 × 10-8 M, and 5 × 10-8 M of 1,25(OH)2D3. After 7, 14, and 21 days of culture, MTT assay for cell viability, alkaline phosphatase activity, and quantification of the percentage of areas with glycosaminoglycans (GAG) stained with periodic acid-Schiff (PAS) were performed. On day 7, all chondrocytes in group 3, that is, those with the highest concentration of plant extract, died. On days 14 and 21, groups 1 and 2 showed a significant reduction in chondrocyte viability compared to the control. At 7, 14, and 21 days, groups 1 and 2 showed significantly lower alkaline phosphatase activity than the control. On day 21, group 2 showed a significant reduction in areas with PAS + GAGs. There were no significant differences between the groups in the expression of gene transcripts for Sox9, Col2, ColX, and aggrecan. The S. glaucophyllum Desf. extract directly affected growing rat chondrocytes by reducing viability, alkaline phosphatase activity, and GAG synthesis without altering the expression of gene transcripts for Sox9, Col2, ColX, and aggrecan, which may be one of the mechanisms by which there is a reduction in bone growth in animals intoxicated by the plant.