根际微生物在促进植物健康中起着至关重要的作用,生产力,和次生代谢产物的积累。目前,对控制社区集合的生态过程的理解有限。为了解决微生物相互作用在根际土壤微生物群的组装和功能中的作用,我们从青藏高原1500公里的Anisodustanguticus收集了根际土壤样本,并对细菌进行了测序,真菌,古细菌,和原生社区。我们观察到根际土壤微生物群落中存在显着但较弱的距离衰减关系。我们对空间的综合分析,非生物,和生物因素表明,原生生物与细菌和真菌之间的营养关系主要影响细菌的α和β多样性,真菌,和Protistan社区,虽然非生物因素对考古群落的影响更大,包括土壤pH值,有效磷,总磷和年平均温度。重要的是,与单个微生物相比,微生物相互作用对Anisodustangutus生理和生态功能的影响更大。网络分析显示,细菌占据共现网络的中心位置,并在该社区中起着至关重要的连接作用。原生生物的加入增加了细菌的稳定性,真菌,和考古网络。总的来说,我们的发现表明,营养关系在根际土壤微生物群的组装和功能中起着重要作用。细菌群落是根际群落中不同微生物王国之间的重要联系。这些发现有助于我们充分利用根际微生物对植物的有益功能,实现生物资源的可持续利用。
Rhizosphere microorganisms play a vital role in enhancing plant health, productivity, and the accumulation of secondary metabolites. Currently, there is a limited understanding of the ecological processes that control the assembly of community. To address the role of microbial interactions in assembly and for functioning of the rhizosphere soil microbiota, we collected rhizosphere soil samples from Anisodus tanguticus on the Tibetan Plateau spanning 1500 kilometers, and sequenced the bacteria, fungi, archaea, and protist communities. We observed a significant but weak distance-decay relationship in the microbial communities of rhizosphere soil. Our comprehensive analysis of spatial, abiotic, and biotic factors showed that trophic relationships between protists and bacteria and fungi predominantly influenced the alpha and beta diversity of bacterial, fungal, and protistan communities, while abiotic factors had a greater impact on archaeal communities, including soil pH, available phosphorus, total phosphorus and mean annual temperature. Importantly, microbial interactions had a more significant influence on Anisodus tanguticus physiological and ecological functions compared to individual microorganisms. Network analyses revealed that bacteria occupy a central position of the co-occurrence network and play a crucial role of connector within this community. The addition of protists increased the stability of bacterial, fungal, and archaeal networks. Overall, our findings indicate that trophic relationships play an important role in assembly and for functioning of the rhizosphere soil microbiota. Bacterial communities serve as a crucial link between different kingdoms of microorganisms in the rhizosphere community. These findings help us to fully harness the beneficial functions of rhizosphere microorganisms for plants and achieve sustainable use of biological resources.