背景:苯甲酸钠(SB)用于日常产品,例如饮料,果汁,酱汁,油,番茄酱,牙膏,漱口水,化妆品,洁齿剂,和医药产品。然而,即使在安全限度内的剂量下,SB也与性腺毒性有关。锌(Zn),另一方面,已被证明可以改善各种生育指数。因此,本研究旨在探讨锌对SB致睾丸毒性的可能改善作用。
方法:动物随机分为对照组,SB,Zn,SB+Zn所有治疗持续28天。
结果:SB治疗导致生殖激素水平紊乱,精子功能,和运动学和雄激素受体(ANDR)的下调。此外,睾丸SOD水平降低,CAT,GSH,Nrf2和HO-1活性以及IL-1β的增加,TNF-α,NF-κB,观察到Caspase3。这些SB诱导的变形在暴露于锌的SB处理的大鼠中得到改善。
结论:我们的研究表明,锌通过调节Nrf2/HO-1/NF-κB信号和ANDR上调来减轻SB诱导的睾丸毒性。
BACKGROUND: Sodium Benzoate (SB) is used in daily products such as drinks, juices, sauces, oils, ketchup, toothpaste, mouthwashes, cosmetics, dentifrices, and pharmaceutical products. However, SB has been implicated in gonadotoxicity even at a dosage within the safe limit. Zinc (Zn), on the other hand, has been shown to improve various fertility indices. Hence, this study was designed to explore the possible ameliorative effect of Zn on SB-induced testicular toxicity.
METHODS: Animals were randomly divided into control, SB, Zn, and SB+Zn. All treatment lasted for 28 days.
RESULTS: SB treatment caused a derangement in reproductive hormone levels, sperm function, and kinematics and a down-regulation of the Androgen receptor (ANDR). Also, a decrease in testicular levels of SOD, CAT, GSH, Nrf2, and HO- 1 activity and an increase in IL-1β, TNF-α, Nf-κB, and Caspase 3 were observed. These SB-induced distortions were ameliorated in SB-treated rats exposed to Zn.
CONCLUSIONS: Our study suggests that zinc abates SB-induced testicular toxicity by modulating Nrf2/HO-1/ Nf-κB signaling and ANDR upregulation.