本研究评估了当前的DNA定量(Quantifiler™Trio,PowerQuant®,Investigator®Quantiplex®Pro和InnoQuant®HYFast)和常染色体STRs扩增试剂盒(GlobalFiler™,PowerPlex®Fusion6C,Investigator®24PlexQS)使用武装冲突中62种退化的骨骼遗骸(岩骨,股骨,胫骨,和牙齿)具有几个参数(常染色体小,大,男性目标,退化指数,退化的可能性,高于分析阈值的等位基因数量,高于随机阈值的等位基因数量,RFU,峰高比,可报告基因座的数量)。通过比较基于样品平均RFU的DNA数量估计的定量结果来确定最佳qPCR/常染色体STR扩增串联。InnoQuant®HYFast是最敏感的套件,扩增试剂盒之间没有显着差异;然而,研究者®24PlexQS在我们的样本中被发现是最敏感的。这就是为什么InnoQuant™和Investigator®24PlexQS被确定为最佳串联。
This research evaluates the current DNA quantification (Quantifiler™ Trio, PowerQuant®, Investigator® Quantiplex® Pro and InnoQuant® HY Fast) and autosomal STRs amplification kits (GlobalFiler™, PowerPlex® Fusion 6 C, Investigator® 24Plex QS) using 62 degraded skeletal remains from armed conflicts (petrous bone, femur, tibia, and tooth) with several parameters (autosomal small, large, and male target, degradation index, probability of degradation, number of alleles above analytical threshold, number of alleles above stochastic threshold, RFU, peak height ratio, number of reportable loci). The best qPCR/autosomal STRs amplification tandem was determined by comparing quantification results by a DNA quantity estimation based on sample average RFU. InnoQuant® HY Fast was the most sensitive kit, and no significative differences were observed among amplification kits; however, Investigator® 24 Plex QS was found to be the most sensitive in our samples. That is why InnoQuant™ and Investigator® 24Plex QS were determined to be the best tandem.