精子的中期保存有助于鱼类精子的生殖管理;然而,没有关于斑点比目鱼精子的信息,Veraspervariegatus.我们旨在确定最佳稀释剂,温度,稀释率,抗生素,和抗氧化剂的精子活力和细胞活力。评估的稀释剂是海鱼林格氏溶液(MFRS),Stein\'ssolution,300mM蔗糖,和300mM葡萄糖(稀释1:1[精子:稀释剂],1:2、1:4和1:10,并在0、2、4和6°C下保存)。新霉素和庆大霉素(100、200、400和800mg/L)和抗氧化剂(Mito-TEMPO[0、25、50、75、100、125、150、175和200µM],还原型谷胱甘肽[0、2、4、6、8和10mM],和海藻糖[0、50、100、150、200和250mM])在精子保存方面进行了评估。斑点大比目鱼精子冷藏的最有效条件是Stein的溶液在2°C下以1:4的稀释比,新霉素800mg/L和250mM海藻糖的组合在60天后显示精子运动性为43%。这些储存条件对于斑点大比目鱼孵化场将是有价值的。
Intermediate-term preservation of sperm assists the reproductive management of fish spermatozoa; however, no information is available on sperm of the spotted halibut, Verasper variegatus. We aimed to identify the optimum diluents, temperatures, dilution ratios, antibiotics, and antioxidants for sperm motility and cell viability. The diluents evaluated were marine fish Ringer’s solution (MFRS), Stein’s solution, 300 mM sucrose, and 300 mM glucose (diluted 1:1 [sperm: diluent], 1:2, 1:4, and 1:10 and stored at 0, 2, 4, and 6 °C). Neomycin and gentamycin (100, 200, 400, and 800 mg/L) and antioxidants (Mito-TEMPO [0, 25, 50, 75, 100, 125, 150, 175, and 200 µM], reduced glutathione [0, 2, 4, 6, 8, and 10 mM], and trehalose [0, 50, 100, 150, 200, and 250 mM]) were assessed in terms of sperm preservation. The most effective condition for cold storage of spotted halibut sperm was Stein’s solution at a dilution ratio of 1:4 at 2 °C, with a combination of neomycin 800 mg/L and 250 mM trehalose that showed spermatozoa motility of > 43% after 60 days. These storage conditions will be valuable for spotted halibut hatcheries.