Abstract：
To assist in the conservation of collared peccary, it is important to strengthen semen processing protocols. The aim of this study was to compare the effects of different commercial extenders (BTS; NUTRIXcell+ and PRIMXcell Ultra) and TRIS + egg yolk on the functional and morphological aspects of collared peccary semen stored at 17 °C for 48 hours. Ten ejaculates obtained by electroejaculation were divided into 4 aliquots and diluted in the respective extenders, then stored in a biological incubator at 17 °C for 12, 24, 36, and 48 hours. The samples were evaluated for kinetic parameters, membrane functionality, membrane integrity, mitochondrial activity, morphology, and sperm-binding capacity. At the end of storage (48 h), promising results were found for motility parameters, with TRIS + egg yolk (71.0 ± 4.6%) being more efficient than NUTRIXcell+ (38.9 ± 10.9%) (P < 0.05) and similar to BTS (42.9 ± 11.9%) and PRIMXcell Ultra (46.8 ± 10.8%). The results for membrane integrity and mitochondrial activity were around ∼30-50%, with TRIS being the only extender to preserve both parameters (58.9 ± 5.3 and 59.2 ± 5.6%) for up to 48 hours, respectively (P < 0.05). Finally, the extenders could guarantee 60% membrane functionality and ∼ 60-70% normal sperm morphology, as well as similar binding capacity among the groups. In conclusion, TRIS + egg yolk is effective in preserving the sperm parameters of collared peccary semen at 17 °C for 48 hours, while PRIMXcell Ultra and BTS are viable alternatives for this purpose.
摘要：
为了协助保护领形野猪，加强精液处理方案很重要。这项研究的目的是比较不同的商业延伸剂（BTS；NUTRIXcell和PRIMXcellUltra）和TRIS蛋黄对在17°C下储存48小时的带领状野菜精液的功能和形态方面的影响。通过电射精获得的十种射精被分成4份，并在各自的补充剂中稀释，然后在17°C的生物培养箱中储存12、24、36和48小时。评估样品的动力学参数，膜功能，膜完整性,线粒体活性，形态学,和精子结合能力。在存储结束时（48小时），对于运动参数，我们发现了有希望的结果，TRIS蛋黄（71.0±4.6％）比NUTRIXcell（38.9±10.9％）更有效（P<0.05），与BTS（42.9±11.9％）和PRIMXcellUltra（46.8±10.8％）相似。膜完整性和线粒体活性的结果约为30-50%，TRIS是唯一能保持这两个参数（58.9±5.3和59.2±5.6％）长达48小时的扩展器，分别为(P<0.05)。最后,延长剂可以保证60%的膜功能和60%-70%的正常精子形态，以及群体之间相似的约束力。总之,TRIS+蛋黄在17°C下可有效保存铃木精液的精子参数48小时，而PRIMXcellUltra和BTS是用于此目的的可行替代品。
