本研究的重点是优化叶绿素提取技术,其中从叶片上的位置切割叶盘,以提高芝麻(SesamumindicumL.)叶片中的叶绿素浓度。30种芝麻基因型,分类为浅绿色(LG),中间绿色(MG),和深绿色(DG)颜料组基于叶着色,是从更大的田间种植种质库中选择的。调查涉及确定最佳土壤植物分析开发(SPAD)值指数测量,定量色素浓度,探索萃取溶剂,并选择合适的叶盘位置。在不同基因型之间观察到叶绿素含量的显着差异,绿色类别,和叶盘位置。将基因型分类为DG,MG,和LG组揭示了叶片外观与叶绿素含量之间的相关性。该研究强调了类胡萝卜素和叶绿素之间的一致关系,表明它们在适应温暖环境中的作用。对叶盘位置的检查显示,沿叶片的叶绿素梯度显着,强调标准化协议的必要性。叶绿素提取实验确定DMSO和96%乙醇,特别是在85°C下孵育10分钟的那些,作为有效的选择。这项建议考虑了成本效益等因素,时间效率,安全,和环境法规,确保一致和简化的提取过程。对于更高的叶绿素提取,以叶尖为重点,建议沿芝麻叶片进行75%的定位,因为这样会不断增加叶绿素含量。此外,我们的检查揭示了深绿色和浅绿色芝麻植物之间叶肉组织叶片内部结构的显着解剖变化,主要与叶绿体密度和色素产生结构有关。我们的发现,因此,提供叶绿素梯度的有见地的知识,并鼓励使用标准化的协议,使研究人员能够完善他们的实验设计,以进行精确和可比的叶绿素测量。推荐的溶剂选择确保植物生理学的可靠结果,生态学,和环境研究。
This study focuses on optimizing chlorophyll extraction techniques, in which leaf discs are cut from places on the leaf blade to enhance chlorophyll concentration in sesame (Sesamum indicum L.) leaves. Thirty sesame genotypes, categorized into light green (LG), middle green (MG), and deep green (DG) pigment groups based on leaf coloration, were selected from a larger pool of field-grown accessions. The investigation involved determining optimal Soil Plant Analysis Development (SPAD) value index measurements, quantifying pigment concentrations, exploring extraction solvents, and selecting suitable leaf disk positions. Significant variations in chlorophyll content were observed across genotypes, greenness categories, and leaf disk positions. The categorization of genotypes into DG, MG, and LG groups revealed a correlation between leaf appearance and chlorophyll content. The study highlighted a consistent relationship between carotenoids and chlorophyll, indicating their role in adaptation to warm environments. An examination of leaf disk positions revealed a significant chlorophyll gradient along the leaf blade, emphasizing the need for standardized protocols. Chlorophyll extraction experiments identified DMSO and 96% ethanol, particularly in those incubated for 10 min at 85 °C, as effective choices. This recommendation considers factors like cost-effectiveness, time efficiency, safety, and environmental regulations, ensuring consistent and simplified extraction processes. For higher chlorophyll extraction, focusing on leaf tips and the 75% localization along the sesame leaf blade is suggested, as this consistently yields increased chlorophyll content. Furthermore, our examination revealed significant anatomical variations in the internal structure of the mesophyll tissue leaves between deep green and light green sesame plants, primarily linked to chloroplast density and pigment-producing structures. Our findings, therefore, provide insightful knowledge of chlorophyll gradients and encourage the use of standardized protocols that enable researchers to refine their experimental designs for precise and comparable chlorophyll measurements. The recommended solvent choices ensure reliable outcomes in plant physiology, ecology, and environmental studies.