在过去的几十年里,几种新出现/重新出现的蚊媒黄病毒导致热带和亚热带公共卫生问题的疾病暴发.由于识别不同黄病毒包膜蛋白的抗体的交叉反应性,血清监测仍然是一个挑战。以前,我们报道了抗前膜(prM)抗体可以通过Westernblot分析区分三种黄病毒感染。在这项研究中,我们的目的是开发一种血清学检测方法,可以从四种血清复合物中区分感染或接触黄病毒,包括登革热(DENV),Zika(ZIKV),西尼罗河(WNV)和黄热病(YFV)病毒,并探讨其在黄病毒流行国家血清监测中的应用。我们采用蛋白质印迹分析,包括六种黄病毒(DENV1,2和4,WNV,ZIKV和YFV)来自四个血清复合物。我们检测了YF-17D疫苗接种者的血清样本,从DENV,已通过RT-PCR或通过中和测定确认的ZIKV和WNV面板。抗prM抗体对DENV的总体敏感性/特异性,ZIKV,WNV,YFV感染/暴露率为91.7%/96.4%,91.7%/99.2%,88.9%/98.3%,和91.3%/92.5%,分别。当测试来自巴西的48个样本时,我们确定了多种黄病毒感染/暴露,包括DENV和ZIKV,DENV和YFV,DENV,ZIKV和YFV。当测试来自菲律宾的50个样本时,我们检测到DENV,ZIKV,和DENV和ZIKV感染,ZIKV血清阳性率为10%,这与ZIKV在亚洲低水平流通的报道一致。一起,这些发现表明,抗prM抗体是一种黄病毒血清复合物特异性标志物,可用于描述多种黄病毒共同传播区域的四种黄病毒感染/暴露情况.
In the past few decades, several emerging/re-emerging mosquito-borne flaviviruses have resulted in disease outbreaks of public health concern in the tropics and subtropics. Due to cross-reactivities of antibodies recognizing the envelope protein of different flaviviruses,
serosurveillance remains a challenge. Previously we reported that anti-premembrane (prM) antibody can discriminate between three flavivirus infections by Western blot analysis. In this study, we aimed to develop a serological assay that can discriminate infection or exposure with flaviviruses from four serocomplexes, including dengue (DENV), Zika (ZIKV), West Nile (WNV) and yellow fever (YFV) viruses, and explore its application for
serosurveillance in flavivirus-endemic countries. We employed Western blot analysis including antigens of six flaviviruses (DENV1, 2 and 4, WNV, ZIKV and YFV) from four serocomplexes. We tested serum samples from YF-17D vaccinees, and from DENV, ZIKV and WNV panels that had been confirmed by RT-PCR or by neutralization assays. The overall sensitivity/specificity of anti-prM antibodies for DENV, ZIKV, WNV, and YFV infections/exposure were 91.7%/96.4%, 91.7%/99.2%, 88.9%/98.3%, and 91.3%/92.5%, respectively. When testing 48 samples from Brazil, we identified multiple flavivirus infections/exposure including DENV and ZIKV, DENV and YFV, and DENV, ZIKV and YFV. When testing 50 samples from the Philippines, we detected DENV, ZIKV, and DENV and ZIKV infections with a ZIKV seroprevalence rate of 10%, which was consistent with reports of low-level circulation of ZIKV in Asia. Together, these findings suggest that anti-prM antibody is a flavivirus serocomplex-specific marker and can be employed to delineate four flavivirus infections/exposure in regions where multiple flaviviruses co-circulate.