革兰氏染色阳性,有氧,杆状应变,YJM1T和YJM12S,与Maebong山隔离,Dogok-dong,江南谷,首尔,大韩民国。菌株YJM1T和YJM12S在5-35°C下表现出生长(最佳,20-30°C)和pH6-9(最佳,pH7)和在0-4%(w/v)NaCl中。菌株YJM1T和YJM12S与以下节杆菌属成员显示出最高的16SrRNA基因序列相似性:A.nanjingensisA33T(98.3%/98.2%相似性),A.WoluwensisNBRC107840T(98.2%/98.1%),A.humicolaKV-653T(97.3%),米曲霉KV-651T(97.3%),和球形A.NBRC12137T(97.2%)。这些菌株在Reasoner的2A上生长良好,营养,Mueller-Hinton,酵母葡萄糖,和葡萄糖蛋白胨肉提取物琼脂。菌株YJM1T的主要极性脂质是磷脂酰甘油,二磷脂酰甘油,和磷脂酰肌醇。菌株YJM1T的主要呼吸醌是MK-9(H2),菌株YJM1T和YJM12S的主要脂肪酸为anteiso-C15:0,anteiso-C17:0,iso-C15:0和iso-C16:0。DNAG+C含量,基于菌株YJM1T的全基因组序列,为68.3mol%。菌株YJM1T与参考菌株之间的平均核苷酸同一性值和数字DNA-DNA杂交值范围为75.0%至92.7%和21.0%至65.3%,分别。菌株YJM1T对枯草芽孢杆菌和大肠杆菌具有抗菌活性。考虑到化学分类学,表型,基因型,和系统发育结果,我们提出菌株YJM1T代表节杆菌属中的一种新物种,并建议命名为节杆菌属。11月。(菌株YJM1T=KACC23300T=JCM36483T)。
Gram-stain-positive, aerobic, rod-shaped strains, YJM1T and YJM12S, were isolated from Maebong Mountain, Dogok-dong, Gangnam-gu,
Seoul, Republic of Korea. Strains YJM1T and YJM12S exhibited growth at 5-35 °C (optimum, 20-30 °C) and pH 6-9 (optimum, pH 7) and in 0-4 % (w/v) NaCl. Strains YJM1T and YJM12S showed highest 16S rRNA gene sequence similarity to the following members of the genus Arthrobacter: A. nanjingensis A33T (98.3 %/98.2 % similarity), A. woluwensis NBRC 107840T (98.2 %/98.1 %), A. humicola KV-653T (97.3 %), A. oryzae KV-651T (97.3 %), and A. globiformis NBRC 12137T (97.2 %). The strains grew well on Reasoner\'s 2A, nutrient, Mueller-Hinton, yeast-dextrose, and glucose-peptone-meat extract agars. The major polar lipids of strain YJM1T were phosphatidylglycerol, diphosphatidylglycerol, and phosphatidylinositol. The primary respiratory quinone of strain YJM1T was MK-9(H2), and the major fatty acids of strains YJM1T and YJM12S were anteiso-C15 : 0, anteiso-C17 : 0, iso-C15 : 0, and iso-C16 : 0. The DNA G+C content, based on the whole genome sequence of strain YJM1T, was 68.3 mol%. Average nucleotide identity values and digital DNA-DNA hybridization values between strain YJM1T and the reference strains ranged from 75.0 to 92.7 % and from 21.0 to 65.3 %, respectively. Strain YJM1T exhibited antimicrobial activity against Bacillus subtilis and Escherichia coli. Considering the chemotaxonomic, phenotypic, genotypic, and phylogenetic results, we propose the strain YJM1T represents a novel species in the genus Arthrobacter and suggest the name Arthrobacter horti sp. nov. (type strain YJM1T=KACC 23300T=JCM 36483T).