塞纳·米尔.(豆科)是世界范围内分布的重要药用植物。塞纳·亚历山德拉娜(S.alexandrina),该属的药用物种,是传统上用于治疗便秘和消化系统疾病的最著名的草药之一。塞纳意大利(S.italica),属的另一种,原产于从非洲到印度次大陆的地区,包括伊朗。在伊朗,这种植物传统上被用作泻药。然而,研究其使用安全性的植物化学信息和药理学报告很少。在目前的研究中,我们比较了意大利沙棘甲醇提取物和阿兰沙棘甲醇提取物的LC-ESIMS代谢物谱,并测量了该属中作为生物标志物的人参皂苷A和B的含量。通过这个,我们能够研究使用S.italica作为泻药如S.alexandrina的可行性。此外,使用基于HPLC的活性谱分析方法对HepG2癌细胞系进行了肝毒性评估,以定位肝毒性成分并评估其使用安全性.有趣的是,结果表明,这些植物的植物化学特征相似,但有一些差异,特别是它们的相对内容。糖基化黄酮类化合物,蒽醌,蒽醌,苯并色烯酮,和二苯甲酮构成两个物种的主要成分。然而,一些差异,特别是在某些化合物的相对量中,被观察到。根据LC-MS结果,在阿列克替里纳和阿利卡中,人参皂甙A的含量分别为1.85±0.095%和1.00±0.38%,分别。此外,在阿列克替利娜和意大利的含量分别为0.41±0.12%和0.32±0.17%,分别。此外,尽管两种提取物在50和100µg/mL的浓度下都显示出明显的肝毒性,它们在较低浓度时几乎无毒。一起来看,根据结果,S.italica和S.alexandrina的代谢物谱显示出许多共同的化合物。然而,进一步的植物化学,药理学,和临床研究是必要的,以检查S.italica作为泻药的有效性和安全性。
Senna Mill. (Fabaceae) is an important medicinal plant distributed worldwide. Senna alexandrina (S. alexandrina), the officinal species of the genus, is one of the most well-known herbal medicines traditionally used to treat constipation and digestive diseases. Senna italica (S. italica), another species of the genus, is native to an area ranging from Africa to the Indian subcontinent, including Iran. In Iran, this plant has been used traditionally as a laxative. However, very little phytochemical information and pharmacological reports investigating its safety of use are available. In the current study, we compared LC-ESIMS metabolite profiles of the methanol extract of S. italica with that of S. alexandrina and measured the content of sennosides A and B as the biomarkers in this genus. By this, we were able to examine the feasibility of using S. italica as a laxative agent like S. alexandrina. In addition, the hepatotoxicity of both species was evaluated against HepG2 cancer cell lines using HPLC-based activity profiling to localize the hepatotoxic components and evaluate their safety of use. Interestingly, the results showed that the phytochemical profiles of the plants were similar but with some differences, particularly in their relative contents. Glycosylated flavonoids, anthraquinones, dianthrones, benzochromenones, and benzophenones constituted the main components in both species. Nevertheless, some differences, particularly in the relative amount of some compounds, were observed. According to the LC-MS results, the amounts of sennoside A in S. alexandrina and S. italica were 1.85 ± 0.095% and 1.00 ± 0.38%, respectively. Moreover, the amounts of sennoside B in S. alexandrina and S. italica were 0.41 ± 0.12 % and 0.32 ± 0.17%, respectively. Furthermore, although both extracts showed significant hepatotoxicity at concentrations of 50 and 100 µg/mL, they were almost non-toxic at lower concentrations. Taken together, according to the results, the metabolite profiles of S. italica and S. alexandrina showed many compounds in common. However, further phytochemical, pharmacological, and clinical studies are necessary to examine the efficacy and safety of S. italica as a laxative agent.