Abstract：
In the present work, a two-dimensional qNMR method for the determination of sennosides was established. Using band-selective HSQC and the cross correlations of the characteristic 10-10\' bonds, we quantified the total amount of the value-determining dianthranoids in five minutes, thus, rendering the method not only fast, but also specific and stability indicating. The validation of the method revealed excellent accuracy (recovery rates of 98.5 to 103%), precision (RSD values of 3.1%), and repeatability (2.2%) and demonstrated the potential of 2D qNMR in the quality control of medicinal plants. In a second method, the use of 2D qNMR for the single analysis of sennosides A, B, and A1 was evaluated with acceptable measurement times (31 min), accuracy (93.8%), and repeatability (5.4% and 5.6%) for the two major purgatives sennoside A and B. However, the precision for sennoside B and A1 was not satisfactory, mainly due to the low resolution of the HSQC signals of the two compounds.
摘要：
在目前的工作中,建立了人参皂苷的二维qNMR测定方法。使用带选择性HSQC和特征10-10键的互相关，我们在五分钟内量化了确定值的二蒽类化合物的总量，因此，渲染方法不仅快速，而且还表明了具体和稳定性。该方法的验证显示出优异的准确性（回收率为98.5至103%），精度（RSD值为3.1%），和重复性（2.2％），并证明了2DqNMR在药用植物质量控制中的潜力。在第二种方法中，使用2DqNMR对人参皂苷A进行单一分析，B,和A1以可接受的测量时间(31分钟)进行评估，准确度（93.8%），和可重复性(5.4%和5.6%)两种主要的泻药皂甙A和B。皂甙B和A1的精度不令人满意，主要是由于两种化合物的HSQC信号的分辨率较低。
