背景:曼尼普尔邦,印度东北部具有独特的丘陵和山谷地区拓扑结构,具有巨大的农业气候变异性,被认为是水稻多样性的中心之一。曼尼普尔邦黑稻品种在形态上表现出广泛的多样性,果皮颜色,晶粒的形状和大小,香气,粘性或非粘性特征,但特征较少。这些品种中的许多,比如那些叫Chakhao的人,由于高花青素而具有多种健康益处,对当地人民特别重要。重要的是分析具有独特等位基因组合的该种质的遗传多样性和种群结构,以用于水稻育种计划。
结果:我们对总可溶性种子蛋白部分进行了表征,不仅对45个本地黑稻品种进行了指纹分析,还评估了它们的遗传相关性。聚类分析主要产生两组,辅以PCoA散点图,确定地理区别。山黑米更加多样化。种群结构分析显示七个亚种群表明高遗传变异性。24条多态性条带的评分范围为127.8至10.3kDa,由四个蛋白质部分组成。三个多肽带分别归因于谷蛋白和醇溶蛋白的已知部分,虽然可以描述白蛋白和球蛋白部分的一个条带,除了其他诊断带。
结论:一些不同的品种是阿穆比,切多肛交,ChipiBuh,阿瑟布,Poireton,布普梅,KothaChahoII.这些品种可用于未来的黑稻育种计划。这可以进一步防止遗传侵蚀和保护知识产权。
BACKGROUND: The state of Manipur, North East India has distinct topology of hill and valley regions with vast agroclimatic variability, being considered as one of the centers of rice diversity. The indigenous Manipur black rice cultivars exhibit wide range of diversity in morphology, pericarp color, shape and size of grain, aroma, glutinous or non-glutinous features but remain less characterised. Many of these cultivars, such as those named Chakhao, are endowed with multiple health benefits due to high anthocyanins, and hold special importance for the local people. It is important to analyse the genetic diversity and population structure for this germplasm with unique allelic combinations to utilize in rice breeding programme.
RESULTS: We characterized total soluble seed protein fractions to not only fingerprint the 45 indigenous black rice cultivars but assess their genetic relatedness. Cluster analyses generated mainly two groups, complemented by PCoA scatter plot ascertaining geographical distinction. The hill black rice were more diverse. The population structure analysis revealed seven subpopulations indicating high genetic variability. The 24 polymorphic bands were scored in the range of 127.8 to 10.3 kDa comprised of four protein fractions. Three polypeptide bands each were ascribed to known fractions of glutelins and prolamins, while one band each could be described for albumin and globulin fractions, besides other diagnostic bands.
CONCLUSIONS: Some diverse cultivars were Amubi, Chedo Anal, Chipi Buh, Athebu, Poireton, BuPu Mui, Kotha Chahao II. These cultivars can be used in future black rice breeding programmes. This can further prevent genetic erosion and protect intellectual property rights.