caspase,作为一种蛋白酶,在真核细胞凋亡中起着至关重要的作用,细胞凋亡的调节,细胞生长,分化,和豁免权。在蛇床子中鉴定caspase基因家族对了解其抗菌研究有很大帮助。在S.schlegelii,我们鉴定了9个caspase基因,包括4种凋亡引发半胱天冬酶(caspase2、caspase8、caspase9和caspase10),四个细胞凋亡执行者(caspase3a,caspase3b,半胱天冬酶6和半胱天冬酶7)和一个炎性执行者(半胱天冬酶1)。caspase3基因在Chr3和Chr8上的复制可能是通过全基因组复制(WGD)事件或其他复杂的进化过程促进的。总的来说,caspase基因的数量在高脊椎动物中相对保守,同时表现出硬骨鱼的变化。此外,同伦分析和系统发育关系分析支持了这些半胱天冬酶基因家族在Schlegelii中的正确分类,尤其是复制的基因.此外,在健康条件下评估了这些caspase基因在S.schlegelii不同组织中的表达模式。结果表明,大多数caspase基因在肠道中的表达水平显着升高,脾,脾还有肝脏.为了进一步研究这些caspase基因在Schlegelii中的潜在免疫功能,我们用A.salmonicida和V.anguillarum挑战个人,分别。沙门氏菌感染后,在整个感染时间点,蛇床子肝脏和脾脏中半胱天冬酶1的表达水平持续升高.大多数caspase家族成员在肠道中的表达水平在V.anguillarum感染后表现出明显的差异。本研究提供了一个全面的了解S.schlegeliicaspase基因家族,从而为进一步研究这些caspase基因的功能作用奠定了坚实的基础。
The caspase, functioning as a proteinase, plays a crucial role in eukaryotic cell apoptosis, regulation of apoptosis, cellular growth, differentiation, and immunity. The identification of caspase gene family in Sebastes schlegelii is of great help to understand its antimicrobial research. In S. schlegelii, we totally identified nine caspase genes, including four apoptosis initiator caspases (caspase 2, caspase 8, caspase 9 and caspase 10), four apoptosis executioners (caspase 3a, caspase 3b, caspase 6, and caspase 7) and one inflammatory executioner (caspase 1). The duplication of caspase 3 genes on chr3 and chr8 may have been facilitated by whole genome duplication (WGD) events or other complex evolutionary processes. In general, the number of caspase genes relatively conserved in high vertebrates, while exhibiting variation in teleosts. Furthermore, syntenic analysis and phylogenetic relationships analysis supported the correct classification of these caspase gene family in S. schlegelii, especially for genes with duplicated copies. Additionally, the expression patterns of these caspase genes in different tissues of S. schlegelii under healthy conditions were assessed. The results revealed that the expression levels of most caspase genes were significantly elevated in the intestine, spleen, and liver. To further investigate the potential immune functions of these caspase genes in S. schlegelii, we challenged individuals with A. salmonicida and V. anguillarum, respectively. After infection with A. salmonicida, the expression levels of caspase 1 in the liver and spleen of S. schlegelii remained consistently elevated throughout the infection time points. The expression levels of most caspase family members in the intestine exhibited significant divergence following V. anguillarum infection. This study provides a comprehensive understanding of the caspase gene families in S. schlegelii, thereby establishing a solid foundation for further investigations into the functional roles of these caspase genes.