Abstract：
Transient Receptor Potential (TRP) channels, essential for sensing environmental stimuli, are widely distributed. Among them, thermosensory TRP channels play a crucial role in temperature sensing and regulation. Sebastes schlegelii, a significant aquatic economic species, exhibits sensitivity to temperature across multiple aspects. In this study, we identified 18 SsTRP proteins using whole-genome scanning. Motif analysis revealed motif 2 in all TRP proteins, with conserved motifs in subfamilies. TRP-related domains, anchored repeats, and ion-transmembrane domains were found. Chromosome analysis showed 18 TRP genes on 11 chromosomes and a scaffold. Phylogenetics classified SsTRPs into four subfamilies: TRPM, TRPA, TRPV, and TRPC. In diverse organisms, four monophyletic subfamilies were identified. Additionally, we identified key TRP genes with significantly upregulated transcription levels under short-term (30 min) and long-term (3 days) exposure at 24 °C (optimal elevated temperature) and 27 °C (critical high temperature). We propose that genes upregulated at 30 min may be involved in the primary response process of temperature sensing, while genes upregulated at 3 days may participate in the secondary response process of temperature perception. This study lays the foundation for understanding the regulatory mechanisms of TRPs responses to environmental stimuli in S. schlegelii and other fishes.
摘要：
瞬态受体电位(TRP)通道,对感知环境刺激至关重要，分布广泛。其中,热感TRP通道在温度传感和调节中起着至关重要的作用。Sebastesschlegelii,一个重要的水生经济物种，在多个方面表现出对温度的敏感性。在这项研究中,我们使用全基因组扫描鉴定了18种SsTRP蛋白.基序分析显示所有TRP蛋白中的基序2，在亚科中具有保守的图案。TRP相关领域，锚定重复，并发现了离子跨膜结构域。染色体分析显示11条染色体上有18个TRP基因和一个支架。系统发育学将SsTRPs分为四个亚家族：TRPM，TRPA,TRPV,和TRPC。在不同的生物体中，确定了四个单系亚家族。此外,我们确定了在24°C(最佳高温)和27°C(临界高温)的短期(30分钟)和长期(3天)暴露下转录水平显著上调的关键TRP基因.我们认为在30分钟上调的基因可能参与温度传感的主要反应过程，而基因在第3天上调可能参与温度感知的次级反应过程。本研究为了解Schlegelii及其他鱼类TRPs对环境刺激的调控机制奠定了基础。
