目的研究造血干细胞移植后眼表的解剖和功能变化。
本研究包括三组患者。第1组:接受异基因造血干细胞移植(HSCT)的患者(n=26)。第2组:HSCT后发生慢性移植物抗宿主病(GvHD)的患者(n=14)。组3:健康受试者(n=20)。对所有受试者进行了完整的眼科检查,包括Schirmer的测试,TBUT(分解时间)测试,牛津量表,OSDI测试,角膜断层扫描,结膜CD8+淋巴细胞检测。
在分支1中(第1组HSCT前后的比较分析),在以下变量中发现了统计学上的显着差异:最佳矫正视力(BCVA)OD(P=0.08),OSDI检验(P=0.003),TBUTOU(ODP=0,OSP=0.0003),OxfordtestOU(ODP=0.01,OSP=0.0049),CD8+淋巴细胞OU(ODP=0.003,OSP=0.01)。在分支机构2(第2组和第3组的比较分析)中,OU中差异有统计学意义(P<0.001)的变量是:BCVA,OSDI测试,Schirmer\'stestOU,TBUT测试,牛津考试,和CD8+淋巴细胞。最后,在分支3中(HSCT后的第1组和第2组之间的比较分析),OU:在OSDI检验中,差异有统计学意义(P<0.001),Schirmer\'stest,和牛津试验OU;TBUT试验OU中P<0.005。
在我们的研究中,在OSDI检验中观察到统计学上显著的变化,TBUT测试,牛津量表,以及HSCT患者CD8+淋巴细胞的检测。与没有GvHD的患者相比,HSCT后发生GvHD的患者的差异更为显着。
To characterize anatomical and functional changes in the ocular surface after hematopoietic stem cell transplantation.
Three groups of patients were included in the study. Group 1: patients who had undergone allogeneic hematopoietic stem cell transplantation (HSCT) (n = 26). Group 2: patients who developed chronic graft versus host disease (GvHD) after HSCT (n = 14). Group 3: healthy subjects (n = 20). A complete ophthalmological examination was undertaken in all subjects, including Schirmer\'s test, TBUT (break-up-time) test, Oxford scale, OSDI test, corneal tomography, and conjunctival CD8+ lymphocyte detection.
In Branch 1 (comparative analysis before and after HSCT in Group 1), statistically significant differences were found in the following variables: best-corrected visual acuity (BCVA) OD (P = 0.08), OSDI test (P = 0.003), TBUT OU (OD P= 0, OS P= 0.0003), Oxford test OU (OD P= 0.01, OS P= 0.0049), and CD8+ lymphocytes OU (OD P= 0.003, OS P= 0.01). In Branch 2 (comparative analysis between Group 2 and 3), the variables with statistically significant differences (P < 0.001) in OU were: BCVA, OSDI test, Schirmer\'s test OU, TBUT test, Oxford test, and CD8+ lymphocytes. Finally, in Branch 3 (comparative analysis between Group 1 after HSCT and Group 2), statistically significant differences (P < 0.001) were found OU: in OSDI test, Schirmer\'s test, and Oxford test OU; and with P < 0.005 in TBUT test OU.
In our study, statistically significant changes were observed in the OSDI test, TBUT test, Oxford Scale, and the detection of CD8+ lymphocytes in patients who underwent HSCT. Differences were more significant in those patients who had developed GvHD after HSCT compared to those without GvHD.