为了评估植物化学成分,抗菌,和calcitrapaL.的连续提取物的抗氧化活性(C.calcitrapa)空中开花部分,他们在体外评估。用分光光度计,517nm处的样品吸光度用于定量清除活性。阴性对照是DPPH。在目前的研究中,使用琼脂孔技术的扩散适用于测量抗菌活性。使用推荐的标准程序进行植物化学分析。C.calcitrapa的甲醇提取物表现出高水平的总酚酸表示为没食子酸(GA),与氯仿相比,含量为(97.25±0.73mgGAE/g),乙酰乙酸酯,和水提物(27.42±0.29、64.25±0.96和17.25±0.73mgGAE/g),分别。此外,与氯仿相比,甲醇提取物的总单宁含量更高(27.52±0.53mgTAE/g),乙酸乙酯,和水提物(12.02±0.55,26.01±0.81和7.35±0.56mgTAE/g),分别,与甲醇提取物所达到的较高含量(425.93±1.27mgRTE/g)相比,水提取物中的类黄酮含量较低(141.10±1.31mgRTE/g)。在骨化梭菌中发现的类黄酮和酚类化合物的羟基基本上清除自由基。甲醇提取物中自由基清除活性最高(IC50=2.82μg/mL),水提取物(IC50=8.03μg/mL),乙酸乙酯提取物(IC50=4.79μg/mL),和氯仿提取物(IC50=6.33μg/mL),与标准清除活性(IC50=2.52μg/mL)相比。降钙素原对革兰氏阴性菌肺炎克雷伯菌的抗菌性能,大肠杆菌,产气肠杆菌,和鲍曼不动杆菌,除了革兰氏阳性菌株溶血葡萄球菌,粪肠球菌,和金黄色葡萄球菌,显示抑制区直径。这项调查的结果证实,钙形虫的空中开花部分对人类感染具有显著的抗菌作用,和植物可以作为重要的抗氧化剂,可用于预防和治疗由氧化应激引起的严重退行性疾病。qPCR显示,以乙酸乙酯植物提取物相对于抗坏血酸作为对照,钙拉虫提取物以显着的折叠(SOD1和SOD2为1.8倍)提高了SOD1和SOD2(细胞氧化标记)。该结果反映了骨化梭菌提取物具有显著的抗氧化活性。
To evaluate the phytochemical composition, antibacterial, and antioxidant activity of successive extracts of Centaurea calcitrapa L. (C. calcitrapa) aerial flowering parts, they were assessed in vitro. Using a spectrophotometer, the sample absorbance at 517 nm was used to quantify the scavenging activity. The negative control was DPPH. In the current study, the diffusion using agar wells technique was adapted to measure antimicrobial activity. Phytochemical analysis was performed using the recommended standard procedures. The methanol extract of C. calcitrapa exhibited high levels of total phenolic acids expressed as gallic acid (GA), measured as (97.25 ± 0.73 mg GAE/g) content compared to the chloroform, acetyl acetate, and aqueous extracts (27.42 ± 0.29, 64.25 ± 0.96, and 17.25 ± 0.73 mg GAE/g), respectively. Additionally, the methanol extract had a higher total tannin (27.52 ± 0.53 mg TAE/g) content compared to the chloroform, ethyl acetate, and aqueous extracts (12.02 ± 0.55, 26.01 ± 0.81, and 7.35 ± 0.56 mg TAE/g), respectively, while the aqueous extract contains a lower percentage of flavonoids (141.10 ± 1.31 mg RTE/g) compared to the higher content achieved by the methanol extract (425.93 ± 1.27 mg RTE/g). The hydroxyl groups of the flavonoid and the phenolic compounds found in C. calcitrapa are essentially scavenging free radicals. Radical scavenging activity was highest in the methanol extract (IC50 = 2.82 μg/mL), aqueous extract (IC50 = 8.03 μg/mL), ethyl acetate extract (IC50 = 4.79 μg/mL), and chloroform extract (IC50 = 6.33 μg/mL), as compared to the standard scavenging activity (IC50 = 2.52 μg/mL). The antibacterial properties of C. calcitrapa against Gram-negative bacterial strains Klebsiella pneumoniae, Escherichia coli, Enterobacter aerogenes, and Acinetobacter baumanii, in addition to Gram-positive strains Staphylococcus haemolyticus, Enterococcus faecalis, and Staphylococcus aureus, revealed inhibition zone diameter. The findings of this investigation establish that the aerial flowering parts of C. calcitrapa have substantial antibacterial action against human infections, and the plant can serve as a significant antioxidant that can be employed to prevent and treat severe degenerative diseases brought on by oxidative stress. qPCR showed that C. calcitrapa extracts elevate both SOD1 and SOD2 (cellular oxidation markers) with remarkable folds (1.8-fold for SOD1 and SOD2) with ethyl acetate plant extract against ascorbic acid as a control. This result reflects that C. calcitrapa extracts have remarkable antioxidant activity.