Abstract：
This study is the first to explore the essential oil of Ononis angustissima Lam. subsp. filifolia Murb., a subspecies growing in the Algerian northeastern Sahara. The chemical composition was evaluated by GC/GC-EIMS. Antioxidant activity was evaluated using two methods. Thirty-four (91.6%) individual components were identified. The main constituents were linalool (12.6%), hexahydrofarnesylacetone (8.4%), β-eudesmol (6.6%), α-cadinol (6.4%) and T-cadinol (6.1%). The findings provide a chemical basis for understanding relationships between North African subspecies, supporting botanical and genetic classification. The oil exhibited moderate scavenging activity against DPPH radicals (IC50 = 102.30 µg/ml) and high activity in the β-carotene bleaching assay (91.346%). Antimicrobial tests revealed effectiveness against Gram-positive bacteria (Staphylococcus aureus ATCC 25923 and ATCC 43300), limited impact on Gram-negative bacteria (Pseudomonas aeruginosa ATCC 27853 and Escherichia coli ATCC 25922), and good inhibition against Aspergillus niger and Scedosporium apiospermum. A notable larvicidal activity was observed against Date Moth, particularly on L2 larvae.
摘要：
本研究是首次探索OnonisangustissimaLam的精油。subsp.filifoliaMurb.,生长在阿尔及利亚撒哈拉沙漠东北部的一个亚种。通过GC/GC-EIMS评价化学组成。使用两种方法评价抗氧化活性。确定了34个（91.6％）单独的组件。主要成分为芳樟醇(12.6%),六氢法呢基丙酮(8.4%),β-eudesmol（6.6%），α-cadinol（6.4%）和T-cadinol（6.1%）。这些发现为理解北非亚种之间的关系提供了化学基础。支持植物学和遗传分类。该油对DPPH自由基具有中等清除活性（IC50=102.30µg/ml），在β-胡萝卜素漂白试验中具有高活性（91.346％）。抗菌试验显示对革兰氏阳性菌（金黄色葡萄球菌ATCC25923和ATCC43300）有效，对革兰氏阴性菌（铜绿假单胞菌ATCC27853和大肠杆菌ATCC25922）的影响有限，对黑曲霉和枯草头孢菌具有良好的抑制作用。对日期蛾观察到明显的杀幼虫活动，特别是在L2幼虫上。
