在水产养殖中,压力会对鱼类生长产生负面影响。多年来,皮质醇激素被认为同时发挥糖皮质激素和盐皮质激素功能。然而,最近的研究表明,11-脱氧皮质酮(DOC)释放的压力可能有助于皮质醇的作用,虽然这个过程仍然被误解。这里,我们通过RNA-seq评估了DOC对生理和早期转录反应的影响。用DOC和/或糖皮质激素(米非司酮)或盐皮质激素(依普利酮)受体拮抗剂治疗幼鱼。随后,收集血浆,cDNA文库是从载体(对照)的g中产生的,DOC,米非司酮,米非司酮与DOC,依普利酮,和依普利酮与DOC组。血浆中的钙和磷酸盐水平发生变化。结果显示,与对照组相比,DOC诱导的914种差异表达转录本(DES),主要与钠离子跨膜转运有关,糖异生,跨膜转运的负调节,和激活先天免疫反应。DOC与依普利酮的比较显示了444个与细胞-细胞连接组织相关的DES,典型的糖酵解,积极调节免疫反应,和钾离子运输。相反,与米非司酮相比,DOC中未检测到DET。这些数据表明,DOC在ill应激反应和离子运输中具有相关作用,这是由盐皮质激素受体差异调节。
In aquaculture, stress can negatively affect fish growth. For years, the cortisol hormone has been thought to play both glucocorticoid and mineralocorticoid functions. Nevertheless, recent research has suggested that 11-deoxycorticosterone (DOC) released during stress could contribute to cortisol actions, though this process is still misunderstood. Here, we evaluated the DOC effects on physiological and early transcriptional responses by RNA-seq. Juvenile rainbow trout were treated with DOC and/or glucocorticoids (mifepristone) or mineralocorticoid (eplerenone) receptor antagonists. Subsequently, plasma was collected, and cDNA libraries were generated from the gills of vehicle (control), DOC, mifepristone, mifepristone with DOC, eplerenone, and eplerenone with DOC groups. Calcium and phosphate levels in plasma were changed. Results revealed 914 differentially expressed transcripts (DETs) induced by DOC compared with control, mainly associated with sodium ion transmembrane transport, gluconeogenesis, negative regulation of transmembrane transport, and activation of innate immune response. DOC versus eplerenone with DOC comparison displayed 444 DETs related to cell-cell junction organization, canonical glycolysis, positive regulation of immune response, and potassium ion transport. Conversely, no DETs were detected in DOC versus mifepristone with DOC comparison. These data suggest that DOC has a relevant role in gill stress response and ion transport, which is differentially regulated by mineralocorticoid receptors.