背景:药用植物Salaciareticulata的根和茎汤是斯里兰卡糖尿病患者及其并发症的本土治疗方法。在糖尿病患者中,晚期糖基化终产物(AGEs)的形成导致许多病理。然而,这种植物的抗蛋白质糖基化特性的文献很少。本研究报道了网状链球菌各种植物部位的抗蛋白质糖基化和自由基清除潜力。
方法:根的热水提取物(2g干粉/50ml),茎,leaf,树枝,和不同浓度的水果(15.6至500.0µg/ml)进行体外抗糖基化和糖基化逆转测定。1,1-二苯基-2-吡啶酰肼(DPPH)测定用于自由基清除性质。
结果:网状链球菌的各个植物部位均显示出抗蛋白质糖基化和自由基清除活性。根的抗糖基化活性的IC50,茎,leaf,树枝,和果实提取物分别为11.92±1.14、35.18±2.79、113.3±1.91、149.59±1.06和1120.37±229.48µg/ml。芦丁的IC50为21.88±2.82μg/ml。根的EC50,茎,树枝,用于糖基化逆转的叶提取物分别为102.09±6.23、116.99±5.82、154.45±5.79和278.78±14.19µg/ml。叶的自由基清除活性的EC50值,茎,根分别为26.4±4.7、9.0±1.2和9.1±1.3。对于所有测试的参数,根具有显著(p<0.05)的高活性。
结论:五层子具有抗糖基化作用,糖基化逆转,和自由基清除活性。除了根和茎,叶子和树枝也可能是抗糖尿病生物活性分子的有用来源。
BACKGROUND: Decoctions of the root and stem of the medicinal plant
Salacia reticulata is an indigenous remedy for diabetics and its complications in Sri Lanka. In diabetics, the formation of advanced glycation end products (AGEs) leads to many pathologies. Nevertheless, the anti-protein-glycation property of this plant is poorly documented. This study reports the anti-protein-glycation and radical scavenging potential of various plant parts of S. reticulata.
METHODS: Hot water extracts (2g dried powder/50 ml) of root, stem, leaf, twigs, and fruits at various concentrations (15.6 to 500.0 µg/ml) were subjected to anti-glycation and glycation reversing assays in vitro. 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay was used for free radical scavenging property.
RESULTS: Various plant parts of S. reticulata showed anti-protein-glycation and free-radical scavenging activities. IC50 for the anti-glycation activity of root, stem, leaf, twigs, and fruit extracts were 11.92 ± 1.14, 35.18 ± 2.79, 113.3 ± 1.91, 149.59 ± 1.06, and 1120.37 ± 229.48 µg/ml respectively. IC50 of Rutin was 21.88 ± 2.82 µg/ml. EC50 of the root, stem, twigs, and leaf extracts for glycation reversing was 102.09 ± 6.23, 116.99 ± 5.82, 154.45 ± 5.79, and 278.78 ± 14.19 µg/ml respectively. The EC50 values for the radical scavenging activity of leaf, stem, and roots were 26.4±4.7, 9.0±1.2, and 9.1±1.3 respectively. Root had significantly (p<0.05) high activity for all the parameters tested.
CONCLUSIONS: Salacia reticulata possess anti-glycation, glycation-reversing, and free radical scavenging activities. Other than root and stem, the leaves and twigs too may be a useful source for anti-diabetic bioactive molecules.