Abstract：
The Salacia reticulata, a medicinal woody climbing shrub, was utilized for our study, the green synthesis of CuO nanoparticles, which were analyzed through SEM, EDX, FTIR, XRD, and UV‒Vis spectroscopy. This study assessed the toxicity to zebrafish embryos and explored the antibacterial, cytotoxic, antidiabetic, and anti-inflammatory properties of the synthesized nanoparticles. In results, the UV absorption of the CuO NPs showed that the intensity of nanoparticle green colloidal suspension changed from blue to green, which also confirmed that the spectrum of the green CuO NPs changed from colorless to black. in FT-IR and XRD spectral analysis to identify functional groups and determine the particle size of CuO NPs prepared by green and chemical methods. Its showed that CuO NPs (green) had a size of approximately 42.2 nm, while CuO NPs (chemical) had a size of approximately 84 nm. The morphology of these NPs was analyzed using SEM-EDX. Compared with their chemically prepared counterparts, the green-synthesized CuO nanoparticles demonstrated superior dispersion. Additionally, both green and chemical CuO nanoparticles at a concentration of 200 µL/mL caused developmental anomalies and increased mortality in zebrafish embryos and larvae. The green and chemical CuO NPs inhibited α-glucosidase enzyme activity at concentrations between 10 and 50 µL/mL, with IC50 values of 22 µL/mL and 26 µL/mL, respectively. The extract exhibited anti-inflammatory activity, with IC50 values of 274 and 109 µL/mL. The authors concluded that this green nanoparticle method has potential as a more eco-friendly and cost-effective alternative to traditional synthetic methods. NPs are widely used in human contact fields (medicine and agriculture), hence synthesis methods that do not involve toxic substances are becoming increasingly important.
摘要：
Salaciareticulata,药用木本攀援灌木，用于我们的研究，CuO纳米粒子的绿色合成，通过扫描电镜分析，EDX,FTIR,XRD,和紫外可见光谱。这项研究评估了对斑马鱼胚胎的毒性，并探索了抗菌，细胞毒性，抗糖尿病药,和合成的纳米颗粒的抗炎特性。在结果中，CuO纳米粒子的紫外吸收表明，纳米粒子绿色胶体悬浮液的强度由蓝色变为绿色，这也证实了绿色CuONPs的光谱从无色变为黑色。在FT-IR和XRD光谱分析中鉴定官能团并确定通过绿色和化学方法制备的CuONPs的粒径。它显示CuONPs（绿色）的尺寸约为42.2nm，而CuONP（化学）的尺寸约为84nm。使用SEM-EDX分析这些NP的形态。与化学制备的同行相比，绿色合成的CuO纳米粒子表现出优异的分散性。此外,绿色和化学CuO纳米颗粒浓度为200µL/mL时都会导致斑马鱼胚胎和幼虫的发育异常和死亡率增加。绿色和化学CuONP在10至50µL/mL的浓度下抑制α-葡萄糖苷酶活性，IC50值分别为22微升/毫升和26微升/毫升，分别。提取物表现出抗炎活性，IC50值为274和109µL/mL。作者得出结论，这种绿色纳米粒子方法具有作为传统合成方法的更环保和更具成本效益的替代方法的潜力。NP广泛应用于人类接触领域(医学和农业)，因此，不涉及有毒物质的合成方法变得越来越重要。
