据报道,婴儿期突然意外死亡(SUDI)中齿状回(DG)的形态差异,具有局灶性颗粒细胞(GC)胆红素(FGCB)的特征,据报道原因不明的SUDI增加,包括婴儿猝死综合症(SIDS),与解释SUDI(ESUDI)相比。然而,这些形态如何相互关联以及它们沿着前后长度的范围还有待确定。这项回顾性研究评估了FGCB的患病率,单个或聚集的异位GC,颗粒细胞分散(GCD),异托邦,超卷积,间隙,变薄,血管夹层(BVD),和袖口(BV袖口),在澳大利亚SUDI队列中,并比较了这些特征在eSUDI和无法解释的SUDI中的患病率。我们分析了来自90例婴儿的850例福尔马林固定石蜡包埋的海马外侧膝状核水平的连续和亚系列切片,并在97%的婴儿中确定了GCD,单个异位细胞,超卷积,变薄,和BVD在60%-80%,36%的异托邦,间隙,9%-15%的异位细胞簇和BV卷曲,FGCB占18%。这些功能集中在3-5个串行部分。FGCB的存在与单异位GC和超卷积相关。在无法解释的SUDI(n=74)和eSUDI(n=16)之间,这些特征的患病率没有差异。我们的发现强调了DG的形态特征是高度局部化的,沿前后长度在其焦点位置延伸14-35µm。因此,沿纵向范围的多个部分需要识别它们。在我们的队列中,没有将SUDI与eSUDI区分开的功能,因此,我们不能断定这些特征中的任何一个是异常的,它们的功能意义还有待确定。
Morphological differences in the dentate gyrus (DG) have been reported in sudden unexpected deaths in infancy (
SUDI), with the feature of focal granule cell (GC) bilamination (FGCB) reported as increased in unexplained
SUDI, including sudden infant death syndrome (SIDS), compared with explained
SUDI (eSUDI). However, it remains to be determined how these morphologies relate to each other and their extent along the anteroposterior length. This retrospective study evaluated the prevalence of FGCB, single or clustered ectopic GCs, granule cell dispersion (GCD), heterotopia, hyperconvolution, gaps, thinning, blood vessel dissection (BVD), and cuffing (BV cuffing), in an Australian SUDI cohort, and compared the prevalence of these features in eSUDI and unexplained
SUDI. We analyzed 850 formalin-fixed paraffin-embedded serial and subserial sections of the hippocampus at the level of the lateral geniculate nucleus from 90 infants, and identified GCD in 97% of infants, single ectopic cells, hyperconvolution, thinning, and BVD in 60%-80%, heterotopia in 36%, gaps, clusters of ectopic cells and BV cuffing in 9%-15%, and FGCB in 18%. These features are clustered within 3-5 serial sections. The presence of FGCB correlated with single ectopic GCs and hyperconvolution. There were no differences in the prevalence of these features between unexplained
SUDI (n = 74) and eSUDI (n = 16). Our findings highlight that DG morphological features are highly localized, extending 14-35 µm at their focal location(s) along the anteroposterior length. Consequently, multiple sections along the longitudinal extent are required to identify them. No feature differentiated SUDI from eSUDI in our cohort, thus we cannot conclude that any of these features are abnormal and it remains to be determined their functional significance.