信号转导和转录激活因子(STAT)家族的成员作为转录激活因子可枢转地发挥功能,整合到炎症反应的调节中。银鱼的水产养殖经常受到外源压力的影响,其中包括热波动,尤其是低温,氧气水平降低,和细菌病原体的攻击。尽管这些压力源的关键影响,科学文献在我们对STAT途径在银鱼适应性反应机制中的作用的理解方面存在显著差距。为了解决这个空白,我们通过全面和系统的生物信息学分析,鉴定了银鱼中命名为Pastat1,Pastat2,Pastat3,Pastat4和Pastat5的stat基因。对基因构型和组成基序的进一步审查已经阐明STAT蛋白具有类似的结构框架并且表现出显著的进化保留。随后,通过RT-qPCR验证了5个stat基因在健康鱼类12个不同组织和4个生长期的表达模式,显示Pastat基因的表达在时间和空间上都是特定的,大多数stat基因在脾脏中以较高的水平表达,跟随肌肉,吉尔,还有肝脏.暴露于外源应激源的转录组学分析,特别是甲醛和低温条件,阐明了Pastat1和Pastat2基因对这些环境挑战表现出更高的敏感性。RT-qPCR分析显示,在这些外源性损伤后,长时间的细菌感染后,PaS中jak1和Pastat基因套件的表达谱发生了显着变化。此外,与先天免疫和细胞凋亡有关的下游效应子的基因表达显示出明显的偏差。这项研究还阐明了Pastat基因家族在调节外源性应激源和随后的病原侵入期间银鱼内的先天免疫应答和凋亡调节中的作用。
Members of the Signal Transducer and Activator of Transcription (
STAT) family function pivotally as transcriptional activators integral to the modulation of inflammatory responses. The aquaculture of silver pomfret is frequently compromised by the imposition of exogenous stressors, which include thermal fluctuations, notably low-temperatures, diminished oxygen levels, and the onslaught of bacterial pathogens. Notwithstanding the critical impact of these stressors, the scientific literature presents a notable gap in our understanding of the STAT pathway\'s role in the silver pomfret\'s adaptive response mechanisms. To address this lacuna, we identified
stat genes in the silver pomfret-denominated as Pastat1, Pastat2, Pastat3, Pastat4, and Pastat5-through a thorough and systematic bioinformatics analysis. Further scrutiny of the gene configurations and constituent motifs has elucidated that
STAT proteins possess analogous structural frameworks and exhibit significant evolutionary preservation. Subsequently, the expression patterns of five
stat genes were verified by RT-qPCR in twelve different tissues and four growth periods in healthy fish, showing that the expression of Pastat genes was temporally and spatially specific, with most of the
stat genes expressed at higher levels in the spleen, following muscle, gill, and liver. Transcriptomic analysis of exposure to exogenous stressors, specifically formaldehyde and low-temperature conditions, elucidated that Pastat1 and Pastat2 genes exhibited a heightened sensitivity to these environmental challenges. RT-qPCR assays demonstrated a marked alteration in the expression profiles of jak1 and Pastat gene suites in PaS upon prolonged bacterial infection subsequent to these exogenous insults. Moreover, the gene expression of the downstream effectors involved in innate immunity and apoptosis displayed marked deviations. This study additionally elucidated the Pastat gene family\'s role in modulating the innate immune response and apoptotic regulation within the silver pomfret during exogenous stressors and subsequent pathogenic incursions.