Abstract：
Signal Transducer and Activator of Transcription (STAT) proteins represent a critical transcription factor family with multifaceted roles in diverse fundamental eukaryotic processes. In Drosophila, STAT exerts a pivotal regulatory influence on oogenesis, governing the early differentiation of follicular cells and ensuring proper encapsulation of germ-line cells. However, the role of STAT in egg development in silkworms remains unknown. In the present study, using CRISPR/Cas9 technology, we successfully generated a strain of silkworms with targeted deletion of the STAT-L gene, which resulted in significant reproductive abnormalities observed in female moths, including shortened fallopian tubes and reduced egg production. The ovaries dissected from STAT-L knockout silkworms during the pupal stage of silkworm exhibited varying degrees of fusion among egg chambers. Additionally, paraffin sections of prepupal ovaries also revealed evidence of egg chambers fusion. To elucidate the molecular mechanism underlying the role of the STAT-L gene regulation on egg development in silkworm, we performed ovarian transcriptomic analysis following STAT-L knockout. Our findings indicated that STAT-L gene can modulate Notch signaling pathway by down-regulating APH-1 gene expression. These results suggest that STAT-L gene plays a crucial role in normal egg chamber formation in silkworms, potentially through its influence on Notch signaling pathway expression.
摘要：
信号转导和转录激活因子（STAT）蛋白代表了一个关键的转录因子家族，在各种基本的真核生物过程中具有多方面的作用。在果蝇中,STAT对卵子发生具有关键的调节作用，控制卵泡细胞的早期分化，并确保生殖细胞的适当封装。然而,STAT在蚕卵发育中的作用尚不清楚。在本研究中,使用CRISPR/Cas9技术，我们成功地产生了一个家蚕菌株，该菌株具有STAT-L基因的靶向缺失，导致在雌蛾中观察到明显的生殖异常，包括缩短输卵管和减少产卵。家蚕p期从STAT-L敲除家蚕解剖的卵巢在卵室之间表现出不同程度的融合。此外,pu前卵巢的石蜡切片也显示了卵室融合的证据。为了阐明STAT-L基因调控对家蚕卵发育的分子机制，我们在STAT-L基因敲除后进行了卵巢转录组分析.我们的发现表明STAT-L基因可以通过下调APH-1基因的表达来调节Notch信号通路。这些结果表明，STAT-L基因在家蚕正常卵室形成中起着至关重要的作用。可能通过其对Notch信号通路表达的影响。
