中国甲鱼(Pelodiscussinensis),是中国经济上重要的水生物种,表现出相当大的性二态性:雄性中华猪大,因此,在市场上更受欢迎。在这项研究中,我们通过使用太平洋生物科学(PacBio)的同工型测序获得了中国黑曲霉的全长(FL)转录组数据,并分析了转录组结构。总的来说,通过单分子实时(SMRT)测序获得1,536,849个高质量的FL转录本,然后使用Illumina测序数据进行校正。接下来,在映射到中华毕赤酵母的参考基因组后,生成了89,666个非冗余FL转录本;使用来自非冗余蛋白质序列数据库(NR)的数据成功地注释了291个融合基因和17,366个新亚型,真核细胞矫形学组(KOG),基因本体论(GO)项目,和KEGG骨科(KO)数据库。此外,19,324个替代的聚腺苷酸化位点,101,625个选择性剪接事件,12,392长非编码RNA,并鉴定了5916个转录因子。Smad4、Wif1和17-β-hsd被鉴定为女性偏向基因,而Nkd2和Prp18在男性中的表达水平高于女性。总之,我们发现男性和女性的中华绒螯蟹个体在AS中存在差异,lncRNA,基因,和成绩单,与Wnt通路有关,卵母细胞减数分裂,和TGF-β途径。Smad4,Wif1和17-β-hsd等女性偏倚基因和Nkd2和Prp18等男性偏倚基因在中华黄曲霉的性别决定中起重要作用。FL转录本是表征中华毕赤酵母转录组的宝贵资源,为进一步研究中华黄芩的性别决定机制奠定基础。
The Chinese soft-shelled turtle (Pelodiscus sinensis), an economically important aquatic species in China, displays considerable sexual dimorphism: the male P. sinensis is larger and, thus, more popular in the market. In this study, we obtained the full-length (FL) transcriptome data of P. sinensis by using Pacific Biosciences (PacBio)\'s isoform sequencing and analyzed the transcriptome structure. In total, 1,536,849 high-quality FL transcripts were obtained through single-molecule real-time (SMRT) sequencing, which were then corrected using Illumina sequencing data. Next, 89,666 nonredundant FL transcripts were generated after mapping to the reference genome of P. sinensis; 291 fusion genes and 17,366 novel isoforms were successfully annotated using data from the nonredundant protein sequence database (NR), eukaryotic orthology groups (KOG), the Gene Ontology (GO) project, and the KEGG Orthology (KO) database. Additionally, 19,324 alternative polyadenylation sites, 101,625 alternative splicing events, 12,392 long noncoding RNAs, and 5916 transcription factors were identified. Smad4, Wif1, and 17-β-hsd were identified as female-biased genes, while Nkd2 and Prp18 held a higher expression level in males than females. In summary, we found differences between male and female P. sinensis individuals in AS, lncRNA, genes, and transcripts, which relate to the Wnt pathway, oocyte meiosis, and the TGF-β pathway. Female-biased genes such as Smad4, Wif1, and 17-β-hsd and male-biased genes such as Nkd2 and Prp18 played important roles in the sex determination of P. sinensis. FL transcripts are a precious resource for characterizing the transcriptome of P. sinensis, laying the foundation for further research on the sex-determination mechanisms of P. sinensis.