PDF(Pubmed)
Abstract：
As a highly economic berry fruit crop, blueberry is enjoyed by most people and has various potential health benefits, many of which are attributed to the relatively high concentrations of flavonoids. To obtain more accurate and comprehensive transcripts, the full-length transcriptome of half-highbush blueberry (Vaccinium corymbosum/angustifolium cultivar Northland) obtained using single molecule real-time and next-generation sequencing technologies was reported for the first time. Overall, 147,569 consensus transcripts (average length, 2738 bp; N50, 3176 bp) were obtained. After quality control steps, 63,425 high-quality isoforms were obtained and 5030 novel genes, 3002 long non-coding RNAs, 3946 transcription factor genes (TFs), 30,540 alternative splicing events, and 2285 fusion gene pairs were identified. To better explore the molecular mechanism of flavonoid biosynthesis in mature blueberry fruit, an integrative analysis of the metabolome and transcriptome was performed on the exocarp, sarcocarp, and seed. A relatively complete biosynthesis pathway map of phenylpropanoids, flavonoids, and proanthocyanins in blueberry was constructed. The results of the joint analysis showed that the 228 functional genes and 42 TFs regulated 78 differentially expressed metabolites within the biosynthesis pathway of phenylpropanoids/flavonoids. O2PLS analysis results showed that the key metabolites differentially accumulated in blueberry fruit tissues were albireodelphin, delphinidin 3,5-diglucoside, delphinidin 3-O-rutinoside, and delphinidin 3-O-sophoroside, and 10 structural genes (4 Vc4CLs, 3 VcBZ1s, 1 VcUGT75C1, 1 VcAT, and 1 VcUGAT), 4 transporter genes (1 VcGSTF and 3 VcMATEs), and 10 TFs (1 VcMYB, 2 VcbHLHs, 4 VcWD40s, and 3 VcNACs) exhibited strong correlations with 4 delphinidin glycosides. These findings provide insights into the molecular mechanisms of flavonoid biosynthesis and accumulation in blueberry fruit.
摘要：
作为一种高度经济的浆果水果作物，大多数人都喜欢蓝莓，具有各种潜在的健康益处，其中许多归因于黄酮类化合物的相对高浓度。为了获得更准确和全面的成绩单，首次报道了使用单分子实时和下一代测序技术获得的半灌木蓝莓（Vaceminiumcorymbosum/angustifolium栽培品种Northland）的全长转录组。总的来说,147,569份共识转录本(平均长度，2738bp；N50，3176bp）获得。质量控制步骤后，获得了63,425个高质量的同工型和5030个新基因，3002长非编码RNA，3946个转录因子基因（TFs），30,540选择性剪接事件，并鉴定出2285个融合基因对。为了更好地探索成熟蓝莓果实黄酮生物合成的分子机制,对外皮进行了代谢组和转录组的综合分析，果肉,和种子。相对完整的苯丙素生物合成途径图，黄酮类化合物，并构建了蓝莓中的原花青素。联合分析结果表明，228个功能基因和42个TFs调控苯丙素/类黄酮生物合成途径中78个差异表达的代谢物。O2PLS分析结果表明，蓝莓果实组织中差异积累的关键代谢产物为albireodelphin，delphinidin3,5-二葡萄糖苷，delphinidin3-O-rutinoside,还有Delphinidin3-O-苦参,和10个结构基因(4个VC4CLs，3VcBZ1s,1个VcUGT75C1,1个VcAT,和1VcUGAT），4个转运蛋白基因（1个VcGSTF和3个VcMATE），和10个TFs(1个VcMYB，2个VcbHLHs,4VcWD40s,和3个VcNACs）与4个菊酯苷表现出很强的相关性。这些发现为蓝莓果实中类黄酮生物合成和积累的分子机制提供了见解。
