肠道菌群失调和亚临床肠道损害在肝硬化中很常见。这项研究的目的是检查肠道损伤生物标志物(二胺氧化酶[DAO],claudin3和肠脂肪酸结合蛋白[I-FABP;FABP2])与肝硬化中肠道微生物群的状态。DAO的血液水平与claudin3,脂多糖(LPS)的血液水平呈负相关,presepsin,TNF-α,和肝硬化的严重程度根据Child-Pugh评分。I-FABP的血液水平与claudin3的血液水平直接相关,而与DAO的血液水平无关。小肠细菌过度生长(SIBO)患者的DAO水平低于无SIBO患者。有无SIBO患者claudin3水平和I-FABP检出率差异无统计学意义。DAO水平与Akkermansiaceae的丰度直接相关,Akkermansia,Allisonella,梭菌科,Dialister,乳酸菌,Muribaculaceae,Negativibacillus,Ruminococus,硫黄螺科,Verrucobiae,和Verrucobiota;它与厌氧菌的丰度成反比,丹毒病菌,和弧菌。I-FABP水平与厌氧菌直接相关,细菌,拟杆菌,双亲,Megamonas,和硒科;它与布鲁氏菌的丰度呈负相关,假竹科,假单胞菌,和弧菌科。claudin3水平与厌氧菌丰度呈正相关,与布鲁氏菌丰度呈负相关,科氏杆菌,蛋黄科,和乳酸菌.
Gut dysbiosis and subclinical intestinal damage are common in cirrhosis. The aim of this study was to examine the association of intestinal damage biomarkers (diamine oxidase [DAO], claudin 3, and intestinal fatty acid binding protein [I-FABP; FABP2]) with the state of the gut microbiota in cirrhosis. The blood levels of DAO were inversely correlated with blood levels of claudin 3, lipopolysaccharide (LPS), presepsin, TNF-α, and the severity of cirrhosis according to Child-Pugh scores. The blood level of I-FABP was directly correlated with the blood level of claudin 3 but not with that of DAO. Patients with small intestinal bacterial overgrowth (
SIBO) had lower DAO levels than patients without
SIBO. There was no significant difference in claudin 3 levels and I-FABP detection rates between patients with and without
SIBO. The DAO level was directly correlated with the abundance of Akkermansiaceae, Akkermansia, Allisonella, Clostridiaceae, Dialister, Lactobacillus, Muribaculaceae, Negativibacillus, Ruminococcus, Thiomicrospiraceae, Verrucomicrobiae, and Verrucomicrobiota; and it was inversely correlated with the abundance of Anaerostipes, Erysipelatoclostridium, and Vibrio. The I-FABP level was directly correlated with Anaerostipes, Bacteroidia, Bacteroidota, Bilophila, Megamonas, and Selenomonadaceae; and it was inversely correlated with the abundance of Brucella, Pseudomonadaceae, Pseudomonas, and Vibrionaceae. The claudin 3 level was directly correlated with Anaerostipes abundance and was inversely correlated with the abundance of Brucella, Coriobacteriia, Eggerthellaceae, and Lactobacillus.