目的:评估短期吸入暴露于牙科复合材料研磨过程中释放的纳米颗粒对氧化应激和抗氧化能力标志物的影响。
方法:24名健康志愿者在牙科车间暴露前后进行检查。在牙科纳米复合材料的研磨过程中,他们在测试室中花费了76.8±0.7分钟。使用个人纳米颗粒采样器(PENS)监测每个参与者呼吸区中的气溶胶颗粒的个体暴露。呼气冷凝液(EBC),血,并在暴露前和暴露后收集尿液样本,以测量一种氧化应激标志物,即,硫代巴比妥酸反应性物质(TBARS),和两个抗氧化能力的生物标志物,即,分光光度法测定三价铁还原抗氧化能力(FRAP)和还原型谷胱甘肽(GSH)。肺活量测定和呼出气一氧化氮(FeNO)用于评估急性吸入暴露的效果。
结果:磨掉的牙科纳米复合材料的平均质量为0.88±0.32g。通过PENS测量的可吸入颗粒和纳米颗粒的平均个体剂量为380±150和3.3±1.3μg,分别。未观察到EBC和血浆中暴露后氧化应激标志物TBARS的显着增加。EBC暴露后抗氧化能力生物标志物FRAP和GSH没有下降,要么。曝光后,在62.5%的志愿者中观察到结膜充血;然而,未观察到肺活量测定或FeNO结果受损.没有发现任何生物标志物与个体暴露的相关性,然而,与干扰因素的几个相关性(年龄,身体质量指数,高血压,血脂异常,和环境污染参数)。
结论:这项研究,在牙科纳米复合材料研磨期间,在志愿者的生物体液中使用氧化应激生物标志物和抗氧化能力生物标志物并没有证明这种强烈的短期暴露有负面影响。然而,需要进一步的研究来评估口腔医师和患者以及不同健康状况人群长期暴露的氧化应激.
OBJECTIVE: To evaluate the effect of short-term inhalational exposure to nanoparticles released during dental composite grinding on oxidative stress and antioxidant capacity markers.
METHODS: Twenty-four healthy volunteers were examined before and after exposure in dental workshop. They spent 76.8 ± 0.7 min in the testing room during grinding of dental nanocomposites. The individual exposure to aerosol particles in each participant´s breathing zones was monitored using a personal nanoparticle sampler (PENS). Exhaled breath condensate (EBC), blood, and urine samples were collected pre- and post-exposure to measure one oxidative stress marker, i.e., thiobarbituric acid reactive substances (TBARS), and two biomarkers of antioxidant capacity, i.e., ferric-reducing antioxidant power (FRAP) and reduced glutathione (GSH) by spectrophotometry. Spirometry and fractional exhaled nitric oxide (FeNO) were used to evaluate the effect of acute inhalational exposure.
RESULTS: Mean mass of dental nanocomposite ground away was 0.88 ± 0.32 g. Average individual doses of respirable particles and nanoparticles measured by PENS were 380 ± 150 and 3.3 ± 1.3 μg, respectively. No significant increase of the post-exposure oxidative stress marker TBARS in EBC and plasma was seen. No decrease in antioxidant capacity biomarkers FRAP and GSH in EBC post-exposure was seen, either. Post-exposure, conjunctival hyperemia was seen in 62.5% volunteers; however, no impairment in spirometry or FeNO results was observed. No correlation of any biomarker measured with individual exposure was found, however, several correlations with interfering factors (age, body mass index, hypertension, dyslipidemia, and environmental pollution parameters) were seen.
CONCLUSIONS: This study, using oxidative stress biomarker and antioxidant capacity biomarkers in biological fluids of volunteers during the grinding of dental nanocomposites did not prove a negative effect of this intense short-term exposure. However, further studies are needed to evaluate oxidative stress in long-term exposure of both stomatologists and patients and diverse populations with varying health statuses.