目的:本研究的目的是阐明IL1R2在LUAD(肺腺癌)中的作用。
背景:IL-1受体家族的特殊成员IL1R2,与IL-1结合并在抑制IL-1途径中起重要作用,这似乎与肿瘤发生有关。新兴研究表明,在几种恶性肿瘤中IL1R2表达水平更高。
目的:在本研究中,我们用免疫组织化学方法评估了IL1R2在LUAD组织中的表达,并探索了各种数据库以确定其是否可能成为潜在的预后生物标志物和治疗靶点.
方法:采用免疫组织化学和UALCAN数据库分析IL1R2在肺腺癌中的表达水平。通过Kaplan-Meier绘图仪确定IL1R2表达与患者预后之间的相关性。IL1R2表达与免疫浸润的相关性由TIMER数据库阐明。通过STRING和Metascape数据库构建并进行蛋白质-蛋白质相互作用网络和基因功能富集分析。
结果:免疫组化显示IL1R2在LUAD患者肿瘤组织中的表达较高,IL1R2水平较低的患者预后较好。我们在几个在线数据库中验证了我们的发现,发现IL1R2基因也与B细胞和中性粒细胞以及CD8+T细胞和耗尽T细胞的生物标志物呈正相关。PPI网络和基因富集分析显示IL1R2的表达也与涉及IL-1信号的复杂的功能特异性网络有关。NF-κB转录因子。
结论:根据这些发现,我们证明IL1R2参与了LUAD的进展和预后,其机制有待进一步研究.
The aim of this study is to figure out the role of IL1R2 in LUAD (lung adenocarcinoma).
IL1R2, a special member of IL-1 receptor family, binds to IL-1 and plays an important role in inhibiting IL-1 pathway, which seems to be involved in tumorigenesis. Emerging studies demonstrated higher IL1R2 expression levels in several malignancies.
In the present study, we assessed the expression of IL1R2 in LUAD tissues with immunohistochemistry and explored various databases to determine whether it could be a potential prognostic biomarker and therapeutic target.
The expression level of IL1R2 in lung adenocarcinoma was analyzed by Immunohistochemistry and UALCAN database. The correlation between IL1R2 expression and the patient prognosis was identified by Kaplan-Meier plotter. The correlation of IL1R2 expression with immune infiltrates was clarified by TIMER database. The protein-protein interaction network and gene functional enrichment analysis were constructed and performed by STRING and Metascape database.
Immunohistochemistry showed that the expression of IL1R2 was higher in tumor tissues of LUAD patients and that patients with lower IL1R2 level have a better prognosis than their counterparts. We validated our findings in several online databases and found that IL1R2 gene was also positively correlated with B cells and neutrophils and biomarkers of CD8+T cells and exhausted T cells. PPI network and gene enrichment analyses showed that expression of IL1R2 was also associated with complex functionspecific networks involving IL-1 signal, NF-KappaB transcription factors.
According to these findings, we demonstrated that IL1R2 was involved in the progression and prognosis of LUAD and the underlying mechanism needs further investigation.