■肠孢子虫,一种强制性的细胞内真菌,在动物和人类中普遍存在。由于它们与人类的密切互动和广泛的区域分布,褐鼠(Rattusnorvegicus)是重要的病原储库。为了评估E.bieneusi的人畜共患传播潜力,对黑龙江省4个城市的817株褐藻进行了分子调查,中国。
■从黑龙江省的四个城市共采集到817株Norvegicus,中国。E.bieneusi的基因分型是通过小亚基核糖体RNA(SSUrRNA)的内部转录间隔区(ITS)片段的PCR扩增进行的。系统发育和相似性分析用于检查E.bieneusi阳性标本的人畜共患潜力和遗传特征。
■在817R.norvegicus中,总感染率为33.3%(272/817)。确定了75种基因型,包括14种已知基因型D(n=167),A(n=15),HLJ-CP1(n=12),WR8(n=6),EbpC(n=2),BEB6(n=1),CS-4(n=1),CHPM1(n=1),河南二号(n=1),HNH-22(n=1),HNH-25(n=1),I(n=1),JLD-XI(n=1),SDD5(n=1),和61个新的基因型命名为SHWR1(n=10),SYSWR1(n=2),和SHWR2到SHWR17,SYSWR2到SYSWR36和QTHWR1到QTHWR8(每个n=1)。此外,10个样本表现出混合基因型感染,包括D+A(n=3),D+EbpC(n=1),D+HLJ-CP1(n=1),D+SHWR1(n=1),D+SHWR16(n=1),D+SHWR17(n=1),SDD5+WR8(n=1),和CS-4+SYSWR36(n=1)。系统发育分析将基因型分为三个主要组:第1组(n=67),第2组(n=5),和第9组(n=3)。
■黑龙江省厌食症的高流行率和遗传多样性表明这些动物传播了病原体。E.bieneusi携带的R.norvegicus可以传播人畜共患疾病,使其对当地人口构成严重危害。因此,必须提高人们对R.norvegicus构成的危险的认识,并采取措施减少其人口,以防止环境污染。
UNASSIGNED: Enterocytozoon bieneusi, an obligatory intracellular fungus, is prevalent among animals and humans. Due to their close interaction with humans and their extensive regional distribution, brown rats (Rattus norvegicus) are important pathogen reservoirs. To assess the zoonotic transmission potential of E. bieneusi, a molecular investigation was conducted on 817 R. norvegicus from four cities in Heilongjiang Province, China.
UNASSIGNED: A total of 817 R. norvegicus were collected from four cities in Heilongjiang Province, China. The genotyping of E. bieneusi was conducted through PCR amplification of the small subunit ribosomal RNA (SSU rRNA)\'s internal transcribed spacer (ITS) segments. Phylogenetic and similarity analyses were used to examine zoonotic potential and genetic characteristics of the E. bieneusi-positive specimens.
UNASSIGNED: Among the 817 R. norvegicus, the total infection rate was 33.3% (272/817). Seventy-five genotypes were identified, including 14 known genotypes D (n = 167), A (n = 15), HLJ-CP1 (n = 12), WR8 (n = 6), EbpC (n = 2), BEB6 (n = 1), CS-4 (n = 1), CHPM1 (n = 1), Henan-II (n = 1), HNH-22 (n = 1), HNH-25 (n = 1), I (n = 1), JLD-XI (n = 1), SDD5 (n = 1), and 61 novel genotypes designated as SHWR1 (n = 10), SYSWR1 (n = 2), and SHWR2 to SHWR17, SYSWR2 to SYSWR36 and QTHWR1 to QTHWR8 (n = 1, each). Moreover, 10 samples exhibited mixed genotype infections, including D + A (n = 3), D + EbpC (n = 1), D + HLJ-CP1 (n = 1), D + SHWR1 (n = 1), D + SHWR16 (n = 1), D + SHWR17 (n = 1), SDD5 + WR8 (n = 1), and CS-4 + SYSWR36 (n = 1). Phylogenetic analysis grouped the genotypes into three main groups: group 1 (n = 67), group 2 (n = 5), and group 9 (n = 3).
UNASSIGNED: The high prevalence and genetic diversity of E. bieneusi in Heilongjiang Province\'s R. norvegicus imply that these animals spread the pathogen. The R. norvegicus that E. bieneusi carries can spread zoonotic disease, making it a serious hazard to the local human population. Therefore, it is imperative to raise awareness about the dangers posed by R. norvegicus and implement measures to reduce their population to prevent environmental contamination.