Rapid detection techniques

  • 文章类型: Journal Article
    海鲜产品被全球消费,消费者对这些产品的质量和安全性的需求越来越大。在自然环境或养殖养殖过程中,一些海鲜很容易受到海洋生物毒素的污染。当人类摄入海鲜中积累的不同毒素时,他们可能会表现出不同的中毒症状。根据调查,有害藻类和其他各种海洋生物产生的海洋毒素主要积聚在海鲜动物的肝脏和消化道等身体器官中。世界上有几个地区报道了生物毒素引起的海产品中毒事件,对人类健康构成威胁。因此,大多数国家已经立法规定了海鲜中这些生物毒素的允许水平。因此,海鲜生产商和供应商有必要在收获前后对海鲜中的毒素进行必要的测试,以禁止含有过量毒素的海鲜进入市场,因此可以减少海产品中毒事件的发生。近年来,一些技术可以快速,方便,灵敏地检测海鲜中的生物毒素,已经开发和验证,这些技术有可能帮助海鲜生产者,供应商和监管机构。本文综述了海鲜毒素的来源和种类,海洋毒素的作用机制和生物累积,以及官方和渔民监督的海鲜中生物毒素的立法和快速检测技术。
    Seafood products are globally consumed, and there is an increasing demand for the quality and safety of these products among consumers. Some seafoods are easily contaminated by marine biotoxins in natural environments or cultured farming processes. When humans ingest different toxins accumulated in seafood, they may exhibit different poisoning symptoms. According to the investigations, marine toxins produced by harmful algal blooms and various other marine organisms mainly accumulate in the body organs such as liver and digestive tract of seafood animals. Several regions around the world have reported incidents of seafood poisoning by biotoxins, posing a threat to human health. Thus, most countries have legislated to specify the permissible levels of these biotoxins in seafood. Therefore, it is necessary for seafood producers and suppliers to conduct necessary testing of toxins in seafood before and after harvesting to prohibit excessive toxins containing seafood from entering the market, which therefore can reduce the occurrence of seafood poisoning incidents. In recent years, some technologies which can quickly, conveniently, and sensitively detect biological toxins in seafood, have been developed and validated, these technologies have the potential to help seafood producers, suppliers and regulatory authorities. This article reviews the seafood toxins sources and types, mechanism of action and bioaccumulation of marine toxins, as well as legislation and rapid detection technologies for biotoxins in seafood for official and fishermen supervision.
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  • 文章类型: Journal Article
    COVID-19的全球大流行迅速增加了许多感染病例和无症状个体的数量,如果不是世界上所有的社会。这个问题增加了对SARS-CoV-2的准确和快速检测的需求。虽然准确和快速的检测对于诊断SARS-CoV-2至关重要,但必须选择适当的治疗过程来帮助患者并防止其进一步传播。SARS-CoV-2的检测平台的准确性和高敏感性和特异性对临床同样重要。区域,和全球竞技场,以减轻二次传播回合。本文的目的是比较当前的检测技术,并介绍适合大流行情况的最准确,最快速的检测技术。因此,首先讨论了快速检测在社会中的重要性。在此之后,目前SARS-CoV-2的快速检测技术被解释并分为三类:基于核酸的,基于蛋白质,和即时护理(PoC)检测测试。然后,讨论了实验室诊断程序的当前问题。最后,还介绍了新技术在对抗COVID-19中的作用,以帮助研究人员开发准确及时的冠状病毒检测。随着冠状病毒继续以有害的方式影响人类生活,快速准确的病毒检测方法的发展可以促进患者护理中的个体和大众人群均可获得COVID-19诊断.在这方面,rRT-PCR和多重RT-PCR检测技术具有前景。
    The global pandemic of COVID-19 has rapidly increased the number of infected cases as well as asymptomatic individuals in many, if not all the societies around the world. This issue increases the demand for accurate and rapid detection of SARS-CoV-2. While accurate and rapid detection is critical for diagnosing SARS-CoV-2, the appropriate course of treatment must be chosen to help patients and prevent its further spread. Testing platform accuracy with high sensitivity and specificity for SARS-CoV-2 is equally important for clinical, regional, and global arenas to mitigate secondary transmission rounds. The objective of this article is to compare the current detection technology and introduce the most accurate and rapid ones that are suitable for pandemic circumstances. Hence, the importance of rapid detection in societies is discussed initially. Following this, the current technology for rapid detection of SARS-CoV-2 is explained and classified into three different categories: nucleic acid-based, protein-based, and point of care (PoC) detection testing. Then, the current issues for diagnostic procedures in laboratories are discussed. Finally, the role of new technologies in countering COVID-19 is also introduced to assist researchers in the development of accurate and timely detection of coronaviruses. As coronavirus continues to affect human lives in a detrimental manner, the development of rapid and accurate virus detection methods could promote COVID-19 diagnosis accessible to both individuals and the mass population at patient care. In this regard, rRT-PCR and multiplex RT-PCR detection techniques hold promise.
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