癌症,一种在不同社会中流行的疾病,在治疗研究中提出了重大挑战。研究表明,联合疗法是可以帮助有效治疗癌症的方法之一。化疗和放射治疗是主要的癌症治疗方法,在这个项目中,联合放化疗治疗,碳纳米管被用作肿瘤化疗的改良载体,以及制备用于局部放射治疗的辐射敏化剂的基质。在合成CNT-铂-姜黄素纳米颗粒(CNT-Pt-CUR)之后,进行了一系列分析以验证这些纳米颗粒的成功生产。技术,如透射电子显微镜(TEM),动态光散射(DLS),紫外-可见光谱,傅里叶变换红外光谱(FTIR),使用X射线衍射(XRD)。表征数据显示,在棒状CNT上具有8.5nm直径的球形Pt纳米颗粒形态,如通过TEM观察到的。此外,FTIR分析证实药物成功加载到纳米颗粒中,强调这种方法在癌症治疗中的潜力。然后,在正常细胞上使用溶血和(3(-4,5-二甲基噻唑-2-基)-2,5-二苯基四唑(MTT)测试来评估CNT-Pt-CUR纳米颗粒的生物相容性。它还探索了这些纳米颗粒在不同浓度下对癌细胞的抗癌功效,有和没有暴露于X射线。该研究证实了这些纳米颗粒的成功合成,并证明了它们对细胞活力的潜在影响。具体来说,当暴露于纳米粒子和X射线时,乳腺癌细胞表现出更高的毒性敏感性.进一步的分析表明,纳米粒子的毒性是剂量依赖性的,用CUR修饰碳纳米管(CNT)纳米颗粒的表面可显着降低血液毒性。有趣的是,纳米粒子的毒性在X射线的存在下显著放大,提示细胞内DNA损伤和活性氧(ROS)水平增加等机制。
Cancer, a prevalent disease across various societies, presents a significant challenge in treatment research. Studies show that combination therapies are one of the methods that can help in the effective treatment of cancer. Chemotherapy and radiation therapy are among the main cancer treatments and in this project, for combined chemoradiotherapy treatment, carbon nanotubes were used as improved carriers of chemotherapy in tumors, as well as a substrate for the preparation of radiation sensitizers for local radiation therapy. Following the synthesis of CNT-Platinum-Curcumin nanoparticles (CNT-Pt-CUR), a series of analyses were conducted to verify the successful production of these nanoparticles. Techniques such as Transmission Electron Microscopy (TEM), Dynamic Light Scattering (DLS), UV-Vis spectroscopy, Fourier Transform Infrared Spectroscopy (FTIR), and X-Ray Diffraction (XRD) were employed. The characterization data revealed a spherical shape Pt nanoparticle morphology with an 8.5 nm diameter on rod-shape CNT, as observed through TEM. Furthermore, FTIR analysis confirmed the successful loaded of the drug into the nanoparticles, highlighting the potential of this approach in cancer treatment. Then, hemolysis and (3(-4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) tests on normal cells were used to assess the biocompatibility of CNT-Pt-CUR nanoparticles. It also explored the anticancer efficacy of these nanoparticles at varying concentrations against cancer cells, both with and without exposure to X-rays. The research confirmed the successful synthesis of these nanoparticles and demonstrated their potential impact on cell viability. Specifically, breast cancer cells exhibited heightened susceptibility to toxicity when exposed to nanoparticles and X-rays. Further analysis revealed that the toxicity of nanoparticles is dose-dependent, and modifying the surface of carbon nanotube (CNT) nanoparticles with CUR significantly reduced blood toxicity. Interestingly, nanoparticle toxicity was significantly amplified in the presence of X-rays, suggesting mechanisms such as DNA damage and increased reactive oxygen species (ROS) levels within cells.