■冠状病毒病-19(COVID-19)是由严重急性呼吸综合征-冠状病毒-2(SARS-CoV-2)引起的疾病。诊断这种感染的最可靠和最广泛接受的方法,尽管面临各种挑战,是逆转录聚合酶链反应(RT-PCR)方法,利用鼻咽拭子样本。与RT-PCR方法相比,逆转录环介导的等温扩增(RT-LAMP)是更简单的核酸扩增方法。这种方法有几个优点,包括:恒温扩增,更快的结果,和潜在更大的考试能力。
■本研究旨在比较使用唾液标本的RT-LAMP方法与使用鼻咽涂片的RT-PCR方法的有效性。
■这是一项横断面设计的分析观察研究。参与者是哈桑·萨迪金总医院COVID-19特殊隔离楼的住院病人,印度尼西亚有可能(covid的临床症状,但未确认NAAT检查)或在2021年9月至2022年2月期间确诊为COVID-19。纳入标准为有症状的COVID-19患者,成人受试者,和复合提及。不能分泌唾液的患者也被排除在外。
■总共,共收集118个标本。用RT-LAMP方法对唾液标本进行的效度检测结果表明,特异性,阳性预测值(PPV)和阴性预测值(NPV),65.5%,100%,100%,75%,分别。在症状发作后3至7天之间接受治疗的受试者的结果增加,即73.2%,100%,100%,82.3%,分别。
在症状发作后3-7天治疗的受试者组中,非常强的特异性伴随着良好的灵敏度和NPV,表明使用唾液样本的RT-LAMP方法可以是检测SARS-CoV-2病毒的有效和可靠的替代工具。
UNASSIGNED: Corona Virus Disease-19 (COVID-19) is a disease caused by Severe-Acute-Respiratory-Syndrome-Coronavirus-2 (SARS-CoV-2). The most reliable and widely accepted method for diagnosing this infection, despite facing various challenges, is the Reverse Transcription Polymerase Chain Reaction (RT-PCR) method, which utilizes nasopharyngeal swab sample. Reverse-transcription loop mediated isothermal amplification (RT-LAMP) is a simpler nucleic acid amplification method compared to the RT-PCR method. This method has several advantages, including: of amplification at constant temperature, faster results, and potentially greater examination capacity.
UNASSIGNED: This study aimed to compare the validity of the RT-LAMP method using saliva specimens with that of the RT-PCR method using nasopharyngeal smears.
UNASSIGNED: This was an analytical observational study with a cross-sectional design. The participants were inpatients in the COVID-19 special isolation building of Hasan Sadikin General Hospital, Indonesia with a probable (clinical symptoms of covid, but not confirm NAAT examination) or confirmed diagnosis of COVID-19 from September 2021 to February 2022. The inclusion criteria are COVID-19 patients with symptoms, adult subjects, and composite mentions. Patients who were unable to secrete saliva were also excluded.
UNASSIGNED: In total, 118 specimens were collected. The validity test results of the saliva specimens using the RT-LAMP method showed sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV), of 65.5%, 100%, 100%, and 75%, respectively. The results increased in subjects treated between 3 and 7 days after symptom onset ie 73.2%, 100%, 100%, and 82.3%, respectively.
UNASSIGNED: The very strong specificity accompanied by good sensitivity and NPV in the group of subjects treated 3-7 days after the onset of symptoms indicates that the RT-LAMP method using saliva specimens can be an efficient and reliable alternative tool in detecting the SARS-CoV-2 virus.