藜麦是一种重要的经济食粮作物。然而,藜麦幼苗易受干旱胁迫,耐旱性的分子机制尚不清楚。在这项研究中,我们比较了在苗期暴露于20%PEG3天和9天的耐旱(L1)和易感(HZ1)基因型的转录组和生理分析。与HZ1相比,干旱胁迫对光合系统的破坏较小,和SOD的含量,L1叶片中POD和CAT含量较高,H2O2和O2-含量较低。基于RNA-seq方法,我们确定了2423、11856、1138和3903(HZ1-C3-VS-T3,HZ1-C9-vs-T9,L1-C3-vs-T3和L1-C9-vs-T9)注释的DEG。Go富集表现在生物过程方面:DEGs参与生物过程,如代谢过程,细胞过程,在所有四个比较处理中,单生物过程最丰富。在分子功能:催化活性的分子功能,结合和转运蛋白活性在所有四个过程中都有最多的DEGs。细胞成分:膜,膜部分,和细胞有最多的DEG在每四个进程。这些DEG包括AP2/ERF,MYB,bHLH,b-ZIP,WRKY,HD-ZIP,NAC,C3h和MADS,编码转录因子。此外,MAPK通路,淀粉和蔗糖代谢,干旱胁迫显著诱导苯丙烷生物合成和植物激素信号转导,其中,G-水解酶-66、G-水解酶-81、G-水解酶-78、Su-合酶-02、Su-合酶-04、Su-合酶-06、BRI1-20和bHLH17在两种基因型的两个干旱胁迫点都下调,PP2C01,PP2C03,PP2C05-PP2C07,PP2C10,F-box01和F-box02在两个基因型的两个干旱胁迫点上调。这些结果与生理反应和RNA-seq结果一致。总的来说,这些发现可能会导致更好地理解耐旱性,本研究中检测到的一些重要DEG可以作为未来研究的目标。本研究结果将为藜麦幼苗抗旱性的分子网络介导提供综合依据,促进藜麦抗旱品种的选育。
Quinoa is an important economic food crop. However,
quinoa seedlings are susceptible to drought stress, and the molecular mechanism of drought tolerance remains unclear. In this study, we compared transcriptomic and physiological analyses of drought-tolerant (L1) and susceptible (HZ1) genotypes exposed to 20% PEG for 3 and 9 days at seedling stage. Compared with HZ1, drought stress had less damage to photosynthetic system, and the contents of SOD, POD and CAT were higher and the contents of H2O2 and O2 -were lower in L1 leaves. Based on the RNA-seq method, we identified 2423, 11856, 1138 and 3903 (HZ1-C3-VS-T3, HZ1-C9-vs-T9, L1-C3-vs-T3 and L1-C9-vs-T9) annotated DEGs. Go enrichment was shown in terms of Biological Process: DEGs involved in biological processes such as metabolic process, cellular process, and single-organism process were most abundant in all four comparison treatments. In Molecular Function: the molecular functions of catalytic activity, binding and transporter activity have the most DEGs in all four processes. Cellular Component: membrane, membrane part, and cell have the most DEGs in each of the four processes. These DEGs include AP2/ERF, MYB, bHLH, b-ZIP, WRKY, HD-ZIP, NAC, C3h and MADS, which encode transcription factors. In addition, the MAPK pathway, starch and sucrose metabolism, phenylpropanoid biosynthesis and plant hormone signal transduction were significantly induced under drought stress, among them, G-hydrolases-66, G-hydrolases-81, G-hydrolases-78, Su-synthase-02, Su-synthase-04, Su-synthase-06, BRI1-20 and bHLH17 were all downregulated at two drought stress points in two genotypes, PP2C01, PP2C03, PP2C05-PP2C07, PP2C10, F-box01 and F-box02 were upregulated at two drought stress points in two genotypes. These results agree with the physiological responses and RNA-seq results. Collectively, these findings may lead to a better understanding of drought tolerance, and some of the important DEGs detected in this study could be targeted for future research. And our results will provide a comprehensive basis for the molecular network that mediates drought tolerance in
quinoa seedlings and promote the breeding of drought-resistant
quinoa varieties.