■镰刀菌枯萎病(FHB)对全球小麦籽粒的产量和品质都有很大影响。宿主抗性是控制FHB的最有效方法,但不幸的是,关于FHB抗性的遗传资源很少;因此,鉴定新的抗性基因或数量性状基因座(QTLs)是有价值的。
■这里,在四个不同的环境中播种了一个重组自交系(RIL)种群,该种群包含来自杂交L661/PI672538的451个品系(2019CZa,2019CZb,2021QL和2021WJ)。
■五个QTL,由两个先前报道的QTL(FhbL693a和FhbL693b)和三个新的QTL(FhbL693c,FhbL693d和FhbL693e),已确定。进一步调查显示,FhbL693b,可以在所有四种环境中检测到FhbL693c和FhbL693d,仅在2019CZb和2021WJ中检测到FhbL693a和FhbL693e,分别。在QTL中,2021WJ的FhbL693d对表型变异效应(PVE)的贡献最大(10.5%),而最小的(1.2%)是2019CZb的FhbL693e和FhbL693a。为FhbL693d选择5Dindel-4,FhbL693c的4Aindel-7和FhbL693b的3Bindel-24使受损小穗的数量减少了2.1,并通过标记辅助选择(MAS)开发了对FHB具有抗性的新品系H140-2。
■这些结果可能有助于将来进一步提高FHB抗性。
UNASSIGNED: Fusarium head blight (FHB) has a large influence on both the yield and quality of wheat grain worldwide. Host resistance is the most effective method for controlling FHB, but unfortunately, very few genetic resources on FHB resistance are available; therefore, identifying novel resistance genes or quantitative trait loci (QTLs) is valuable.
UNASSIGNED: Here, a recombinant inbred line (RIL) population containing 451 lines derived from the cross L661/PI672538 was sown in four different environments (2019CZa, 2019CZb, 2021QL and 2021WJ).
UNASSIGNED: Five QTLs, consisting of two previously reported QTLs (FhbL693a and FhbL693b) and three new QTLs (FhbL693c, FhbL693d and FhbL693e), were identified. Further investigation revealed that FhbL693b, FhbL693c and FhbL693d could be detected in all four environments, and FhbL693a and FhbL693e were detected only in 2019CZb and 2021WJ, respectively. Among the QTLs, the greatest contribution (10.5%) to the phenotypic variation effect (PVE) was FhbL693d in 2021WJ, while the smallest (1.2%) was FhbL693e and FhbL693a in 2019CZb. The selection of 5Dindel-4 for FhbL693d, 4Aindel-7 for FhbL693c and 3Bindel-24 for FhbL693b decreased the number of damaged spikelets by 2.1, and a new line resistant to FHB named H140-2 was developed by marker-assisted selection (MAS).
UNASSIGNED: These results could help to further improve FHB resistance in the future.