背景:石榴,俗称石榴,以其健康益处而闻名,主要与水果和种子的消耗有关。然而,其不可食用的部分,包括树叶,已在传统医学中用作具有抗炎和抗糖尿病特性的药物。考虑到生物活性化合物的丰度,主要是黄酮醇,黄酮,和单宁石榴叶(PGL)提取物具有作为健康促进剂的潜力。然而,它对长寿和健康的影响在很大程度上仍未被探索。
目的:我们的研究旨在探索PGL提取物在秀丽隐杆线虫中增强健康和改善与年龄相关的虚弱的潜力。此外,我们试图阐明其对与应激抗性和寿命相关的分子信号网络的影响.
方法:在通过NMR光谱表征提取物代谢物分布后,进行了表型和应激分析。为了建立分子作用机制,通过实时定量PCR(RT-qPCR)和使用转基因菌株(MIR13,MAH240,LD1和OH16024)研究了长寿关键信号通路的参与.此外,PGL对代谢和脂质积累的影响,以及线粒体稳态,被检查过。
结果:PGL提取物的补充显着增强了抗逆性,并延长了秀丽隐杆线虫的寿命。此外,它改善了运动能力,以及代谢和线粒体功能,表明健康状况总体改善。这些分子机制突出了应激反应的协调调控,代谢稳态,和长寿信号通路。具体来说,我们的结果证明了HLH-30/TFEB的重要作用,结合DAF-16/FOXO和SKN-1/NRF2,作为PGL提取物对健康影响的介体。
结论:我们的发现强调了PGL提取物改善与年龄相关的下降的潜力,诱导长寿,进一步提高健康。鉴于与压力适应相关的分子网络的不同影响,长寿和代谢控制,PGL提取物可能成为一种有前途的天然产物,对老年学领域尤为重要。
BACKGROUND: Punica granatum L., commonly known as pomegranate, is renowned for its health benefits, primarily associated with the consumption of its fruit and seeds. However, its non-edible parts, including leaves, have been used in traditional medicine as a remedy with anti-inflammatory and anti-diabetic properties. Considering the abundance of bioactive compounds, predominantly flavonols, flavones, and tannins P. granatum leaf (PGL) extract holds potential as health-promoting agent. Yet, its effect on longevity and healthspan remains largely unexplored.
OBJECTIVE: Our study aims to explore the potential of PGL extract to enhance healthspan and ameliorate age-related frailty in Caenorhabditis elegans. Additionally, we seek to elucidate its effect on the molecular signaling networks associated with stress resistance and longevity.
METHODS: After characterizing the extract metabolite profile by NMR spectroscopy, phenotypic and stress analyses were performed. In order to establish the molecular mechanism of action, the involvement of signaling pathways key to longevity were investigated by means of real-time quantitative PCR (RT-qPCR) and the use of transgenic strains (MIR13, MAH240, LD1, and OH16024). In addition, the effect of PGL on metabolism and lipid accumulation, as well as mitochondrial homeostasis, was examined.
RESULTS: The PGL extract supplementation significantly enhanced stress resistance and extended the lifespan of C. elegans. Additionally, it improved locomotion, as well as metabolic and mitochondrial functions, indicating an overall improvement in health. The molecular mechanisms highlight the coordinated regulation of stress response, metabolic homeostasis, and longevity signaling pathways. Specifically, our results demonstrate the essential roles of HLH-30/TFEB, in conjunction with DAF-16/FOXO and SKN-1/NRF2, as mediators of the PGL extract effect on healthspan.
CONCLUSIONS: Our findings emphasize the potential of PGL extract to ameliorate age-related decline, induce longevity and further enhance healthspan. Given the diverse effects on the molecular network associated with stress-adaptations, longevity and metabolic control, PGL extract might become a promising natural product with a particular importance to the field of gerontology.