PDF(Pubmed)
Abstract：
UNASSIGNED: Natural colorants, including natural pigments, e.g., anthocyanins, carotenoids, and chlorophylls, in novel and attractive food matrixes have become a popular trend. They impart favorite colors to food products and provide significant therapeutic effects. This study is aimed at extracting and identifying some natural pigments from different plant sources and evaluating their ability as antibacterial, antioxidant, and anticancer activities.
UNASSIGNED: The anthocyanin-rich extract (ARE) is derived from three natural plant sources: pomegranate peel (Punica granatum), chili pepper fruit (Capsicum annuum), and Bougainvillea flowers. Bougainvillea spectabilis are analyzed for biochemical composition, as well as antioxidant, antibacterial, and anticancer activity, HPLC, DPPH, FRAP, disc diffusion assay, MIC, MTT, VEGFR-2, and caspase-9 assays.
UNASSIGNED: All three extracts had varying total phenolic contents, ranging from 14 to 466 mg GAE/g extract, where Punica granatum was the highest (466 mg GAE/g extract), followed by Bougainvillea spectabilis (180 mg GAE/g extract), and then Capsicum annuum (14 mg GAE/g extract). The antioxidant activity rose steadily with raising concentration. The ARE of pomegranate peels recorded highest value, followed by Bougainvillea flowers and chili pepper fruit. The MTT assay revealed an inhibitory action of the tested extracts on the proliferation of HCT-116, MCF-7, and HepG2 in a concentration-based manner. Gene expression of caspase-9 transcripts was considerably multiplied by the application of ARE of pomegranate peels. All the tested extracts inhibited VEGFR-2, and the inhibition (%) expanded gradually with increasing concentrations, achieving the highest value (80 %) at 10 μg/mL. The ARE of pomegranate peels scored highest antibacterial activity, followed by ARE of chili pepper fruit and Bougainvillea flowers. The inhibition zone diameter escalated gradually with rising concentrations of the tested samples.
UNASSIGNED: The AREs of the three studied plant sources can be used as multifunctional products with antioxidant, anticancer, and antibacterial activities that are natural, safe, and cheap.
UNASSIGNED: The anthocyanin-rich extract (ARE) is derived from three natural plant sources: pomegranate peel (Punica granatum), chili pepper fruit (Capsicum annuum), and Bougainvillea flowers. Bougainvillea spectabilis are analyzed for biochemical composition, as well as antioxidant, antibacterial, and anticancer activity, HPLC, DPPH, FRAP, disc diffusion assay, MIC, MTT, VEGFR-2, and caspase-9 assays.
UNASSIGNED: All three extracts had varying total phenolic contents, ranging from 14 to 466 mg GAE/g extract, where Punica granatum was the highest (466 mg GAE/g extract), followed by Bougainvillea spectabilis (180 mg GAE/g extract), and then Capsicum annuum (14 mg GAE/g extract). The antioxidant activity rose steadily with raising concentration. The ARE of pomegranate peels recorded highest value, followed by Bougainvillea flowers and chili pepper fruit. The MTT assay revealed an inhibitory action of the tested extracts on the proliferation of HCT-116, MCF-7, and HepG2 in a concentration-based manner. Gene expression of caspase-9 transcripts was considerably multiplied by the application of ARE of pomegranate peels. All the tested extracts inhibited VEGFR-2, and the inhibition (%) expanded gradually with increasing concentrations, achieving the highest value (80 %) at 10 μg/mL. The ARE of pomegranate peels scored highest antibacterial activity, followed by ARE of chili pepper fruit and Bougainvillea flowers. The inhibition zone diameter escalated gradually with rising concentrations of the tested samples.
UNASSIGNED: The AREs of the three studied plant sources can be used as multifunctional products with antioxidant, anticancer, and antibacterial activities that are natural, safe, and cheap.
摘要：
■天然着色剂，包括天然色素,例如，花青素,类胡萝卜素，和叶绿素，新颖而诱人的食物基质已成为一种流行趋势。它们赋予食品最喜欢的颜色并提供显著的治疗效果。本研究旨在从不同植物来源中提取和鉴定一些天然色素，并评估其抗菌能力，抗氧化剂，和抗癌活动。
■富含花青素的提取物（ARE）来自三种天然植物来源：石榴皮（石榴皮），辣椒果实（辣椒），和九重葛花。分析了九重葛的生化成分，以及抗氧化剂，抗菌，和抗癌活性，HPLC,DPPH,FRAP,圆盘扩散测定，MIC,MTT,VEGFR-2和胱天蛋白酶-9测定。
■这三种提取物的总酚含量各不相同，范围从14到466毫克GAE/克提取物，石榴最高(466毫克GAE/克提取物)，其次是九重葛（180毫克GAE/克提取物），然后是辣椒（14mgGAE/g提取物）。随着浓度的升高，抗氧化活性稳步上升。石榴皮的ARE记录最高值，其次是九重葛花和辣椒果实。MTT测定显示受试提取物以基于浓度的方式对HCT-116、MCF-7和HepG2的增殖具有抑制作用。通过应用石榴皮的ARE，caspase-9转录本的基因表达大大增加。所有受试提取物均对VEGFR-2有抑制作用,且抑制作用(%)随浓度增加而逐渐扩大,在10μg/mL时达到最高值(80%)。石榴皮的ARE抗菌活性最高，其次是辣椒果实和九重葛花。随着测试样品浓度的升高，抑制区直径逐渐增加。
■所研究的三种植物来源的ARE可用作具有抗氧化剂的多功能产品，抗癌，和天然的抗菌活性，安全,而且便宜。
■富含花青素的提取物（ARE）来自三种天然植物来源：石榴皮（石榴皮），辣椒果实（辣椒），和九重葛花。分析了九重葛的生化成分，以及抗氧化剂，抗菌，和抗癌活性，HPLC,DPPH,FRAP,圆盘扩散测定，MIC,MTT,VEGFR-2和胱天蛋白酶-9测定。
■这三种提取物的总酚含量各不相同，范围从14到466毫克GAE/克提取物，石榴最高(466毫克GAE/克提取物)，其次是九重葛（180毫克GAE/克提取物），然后是辣椒（14mgGAE/g提取物）。随着浓度的升高，抗氧化活性稳步上升。石榴皮的ARE记录最高值，其次是九重葛花和辣椒果实。MTT测定显示受试提取物以基于浓度的方式对HCT-116、MCF-7和HepG2的增殖具有抑制作用。通过应用石榴皮的ARE，caspase-9转录本的基因表达大大增加。所有受试提取物均对VEGFR-2有抑制作用,且抑制作用(%)随浓度增加而逐渐扩大,在10μg/mL时达到最高值(80%)。石榴皮的ARE抗菌活性最高，其次是辣椒果实和九重葛花。随着测试样品浓度的升高，抑制区直径逐渐增加。
■所研究的三种植物来源的ARE可用作具有抗氧化剂的多功能产品，抗癌，和天然的抗菌活性，安全,而且便宜。
