目的是确定在肉鸡日粮中使用不同的油料餐时,植酸酶对前盲肠氨基酸(AA)消化率和植酸(InsP6)分解的影响。该研究包括14种饮食:玉米豆粕(SBM)基础饮食和6种含有SBM的饮食,菜籽粕(RSM),和葵花籽粉(SFM),含2种含量,以玉米淀粉为代价(150和300g/kgSBM或SFM,或100和200克/千克RSM)。每种饮食与或不与1,500FTU/kg的植酸酶补充剂混合。在第21天,从回肠的后半部分收集Digesta。平均必需AA消化率,通过回归方法计算,不含植酸酶和含植酸酶的比例分别为84%和85%(SBM),74%和77%(SFM),和66%和73%(RSM),分别。在饮食中,由于饮食中还存在其他蛋白质来源,植酸酶对AA消化率的影响较低,但意义重大。前盲肠InsP6的消失受到油籽粕之间相互作用的显着影响,包含级别,和植酸酶的补充。总的来说,SBM饮食中InsP6的前期消失(52%)高于SFM饮食(38%),而RSM饮食中的中期消失(43%)。跨饮食,随着日粮中InsP6浓度达到12g/kgDM,植酸酶补充对前盲肠InsP6降解的影响线性增加。线性的唯一例外是饮食中含有高SFM,其中含有15.9gInsP6/kgDM。在回肠内容中,肌醇六磷酸酶的补充显着增加了肌醇的浓度,这种效果在含有SBM作为唯一油料粕的饮食中最高。补充植酸酶增加了低级肌醇磷酸的浓度,Ins(1,2,3,4)P4和肌醇四磷酸酯的这种作用最为显着。研究表明,植酸酶对AA消化率的影响在3种测试的油料餐中有所不同,但是这些差异在包含这些膳食的饮食中无法检测到。尽管植酸酶对InsP6及其降解产物回肠含量的影响很大,它们与对AA消化率的影响无关。
The objective was to determine phytase effects on prececal amino acid (AA) digestibility and phytate (InsP6) breakdown when different oilseed meals were used in broiler chicken diets. The study included 14 diets: a corn-soybean meal (SBM) basal diet and 6 diets that contained SBM, rapeseed meal (RSM), and sunflower meal (SFM) with 2 inclusion levels at the expense of corn starch (150 and 300 g/kg SBM or SFM, or 100 and 200 g/kg RSM). Each diet was mixed with or without a phytase supplement of 1,500 FTU/kg. Diets were provided to broilers for 5 D. Digesta from the posterior half of the ileum were collected on day 21. The average essential AA digestibility, calculated by a regression approach, without and with phytase was 84 and 85% (SBM), 74 and 77% (SFM), and 66 and 73% (RSM), respectively. In the diets, phytase effects on AA digestibility were lower owing to other protein sources also present in the diet, but significant. Prececal InsP6 disappearance was significantly affected by interactions between oilseed meal, inclusion level, and phytase supplementation. Overall, prececal InsP6 disappearance was higher in SBM diets (52%) than in SFM diets (38%) and intermediate in RSM diets (43%). Across diets, phytase supplementation effects on prececal InsP6 degradation linearly increased with the InsP6 concentration of the diet up to 12 g/kg DM. The only exception from linearity was the diet with the high inclusion of SFM, which contained 15.9 g InsP6/kg DM. In the ileal content, the concentration of myo-inositol was significantly increased by phytase supplementation, and this effect was highest in the diets that contained SBM as the only oilseed meal. Concentrations of lower inositol phosphates were increased by phytase supplementation, and this effect was most remarkable for Ins(1,2,3,4)P4 and inositol tetrakisphosphates. The study showed that phytase effects on AA digestibility varied among the 3 tested oilseed meals, but these differences were not detectable in the diets containing these meals. Although phytase effects on ileal content of InsP6 and its degradation products were substantial, they were not related to the effects on AA digestibility.