PDF(Pubmed)
Abstract：
The OVATE gene family plays an important role in regulating the development of plant organs and resisting stress, but its expression characteristics and functions in sorghum have not been revealed. In this study, we identified 26 OVATE genes in the sorghum BTx623 genome, which were divided into four groups and distributed unevenly across 9 chromosomes. Evolutionary analysis showed that after differentiation between sorghum and Arabidopsis, the OVATE gene family may have experienced unique expansion events, and all OVATE family members were negatively selected. Transcriptome sequencing and RT-qPCR results showed that OVATE genes in sorghum showed diverse expression characteristics, such as gene SORBl_3001G468900 and SORBl_3009G173400 were significantly expressed in seeds, while SORBI_3005G042700 and SORBI_3002G417700 were only highly expressed in L1. Meantime, in the promoter region, a large number of hormone-associated cis-acting elements were identified, and these results suggest that members of the OVATE gene family may be involved in regulating specific development of sorghum leaves and seeds. This study improves the understanding of the OVATE gene family of sorghum and provides important clues for further exploration of the function of the OVATE gene family.
摘要：
OVATE基因家族在调节植物器官发育和抵抗逆境胁迫中起着重要作用,但其在高粱中的表达特征和功能尚未揭示。在这项研究中,我们在高粱BTx623基因组中鉴定出26个OVATE基因，分为四组，在9条染色体上分布不均。进化分析表明,高粱与拟南芥分化后,OVATE基因家族可能经历了独特的扩增事件，所有OVATE家族成员均为阴性选择。转录组测序和RT-qPCR结果表明,高粱OVATE基因表现出多样化的表达特征,如SORBl_3001G468900和SORBl_3009G173400基因在种子中显著表达,而SORBI_3005G042700和SORBI_3002G417700仅在L1中高表达。同时,在启动子区域，大量的激素相关的顺式作用元件被确定，这些结果表明，OVATE基因家族的成员可能参与调节高粱叶片和种子的特定发育。本研究提高了对高粱OVATE基因家族的认识,为进一步探索OVATE基因家族的功能提供了重要线索。
