背景:转基因事件DAS44406-6-E3-使大豆成为除草剂(草甘膦,2,4-D和草铵膦)和毛毛虫抗性。E3大豆在2021/2022年在巴西收获时进行了商业发行。我们进行了这项研究,以测试草甘膦(Gly)和2,4-D单独应用和在商业混合物中是否会影响亚洲大豆锈病(ASR)。通过分离的叶子进行测定,在体内,在受控环境中,使用除草剂Gly,2,4-D,和Gly+2,4-D,和病原体接种。疾病严重程度,并对孢子产量进行了评价。
结果:只有除草剂Gly和Gly2,4-D在离体叶片和体内引起抑制性ASR。在体内预防性和治疗性应用时,这些除草剂降低了疾病的严重程度和真菌的孢子产生。在体内,对疾病严重程度的抑制对于Gly+2,4-D达到87%,对于Gly达到42%。观察到商业Gly+2,4-D混合物的协同作用。在体内测定中单独应用2,4-D没有降低或增加严重性。Gly和Gly+2,4-D在抑制疾病方面具有残余作用。种植E3大豆可以结合杂草和毛毛虫管理益处和ASR抑制。
结论:在抗性E3大豆中应用Gly和Gly2,4-D除草剂对ASR具有抑制活性。
BACKGROUND: Transgenic event DAS44406-6 (E3) makes soybeans that are herbicide [glyphosate (Gly), 2,4-dichlorophenoxyacetic acid (2,4-D) and glufosinate] and caterpillar resistant. The E3 soybean was commercially released for the 2021/2022 harvest in Brazil. We conducted this study to test whether Gly and 2,4-D applied alone and in a commercial mixture affect Asian soybean rust (ASR). Assays were conducted in detached leaves and in vivo, in a controlled environment using the herbicides Gly, 2,4-D and Gly + 2,4-D, and pathogen inoculation. Disease severity and spore production were evaluated.
RESULTS: Only the herbicides Gly and Gly + 2,4-D inhibited ASR in detached leaves and in vivo. When applied preventively and curatively in vivo, these herbicides reduced the disease severity and spore production of the fungus. In vivo, inhibition of disease severity reached 87% for Gly + 2,4-D and 42% for Gly. A synergistic effect was observed with the commercial Gly + 2,4-D mixture. Application of 2,4-D alone in the in vivo assays did not reduce or increase disease severity. Gly and Gly + 2,4-D act residually in inhibiting the disease. Growing E3 soybeans may combine weed and caterpillar management benefits with ASR inhibition.
CONCLUSIONS: Application of Gly and Gly + 2,4-D herbicides in resistant E3 soybean shows inhibitory activity for ASR. © 2023 Society of Chemical Industry.