目前的研究检查了芳樟醇在预防术后腹部粘连中的作用。20只雄性Wistar大鼠随机分为4组。(1)假:在这个群体中,接近腹部,没有任何操纵,缝合了.(2)对照:该组大鼠行外科手术以诱导粘连。这包括在右腹侧切开三个切口,并在左腹侧切除一块1×1厘米的腹膜。(3)治疗组:这些组接受与对照组相同的手术程序以引起粘连。这些组中的动物口服芳樟醇,剂量为50和100mg/kg,分别,为期14天。此外,假手术组和对照组大鼠接受生理盐水灌胃14天。TNF-α的评价,TGF-β,VEGF,使用蛋白质印迹和IHC方法进行caspase3。此外,氧化应激生物标志物,如MDA,TAC,GSH,在腹膜粘连组织中评估NO。结果显示芳樟醇通过降低TNF-α显著减少腹膜粘连,TGF-β,VEGF,和胱天蛋白酶3水平。此外,MDA浓度显著降低,虽然没有,TAC,GSH水平显著升高。总的来说,芳樟醇可有效防止粘连形成和减少炎症,血管生成,凋亡,和氧化应激。因此,芳樟醇作为有效的抗氧化剂被建议用于减少大鼠术后粘连。
The current study examines the effects of linalool in preventing postoperative abdominal adhesions. Twenty male Wistar rats were randomly divided into four groups. (1) Sham: in this group, the abdomen was approached, and without any manipulations, it was sutured. (2) Control: rats in this group underwent a surgical procedure to induce adhesions. This involved making three incisions on the right abdominal side and removing a 1×1-cm piece of the peritoneum on the left abdominal side. (3) Treatment groups: these groups underwent the same surgical procedure as the control group to induce adhesions. Animals in these groups received linalool orally with doses of 50 and 100 mg/kg, respectively, for a period of 14 days. Moreover, rats in the sham and control groups received normal saline via gavage for 14 days. The evaluation of TNF-α, TGF-β, VEGF, and caspase 3 was performed using western blot and IHC methods. Furthermore, oxidative stress biomarkers such as MDA, TAC, GSH, and NO were assessed in the peritoneal adhesion tissue. The findings revealed that linalool significantly reduced peritoneal adhesions by reducing TNF-α, TGF-β, VEGF, and caspase 3 levels. Moreover, MDA concentration was significantly decreased, while NO, TAC, and GSH levels were notably increased. Overall, linalool was effective in preventing adhesion formation and reduced inflammation, angiogenesis, apoptosis, and oxidative stress. Therefore, linalool as a potent antioxidant is suggested for reducing postoperative adhesions in rats.