目的:观察骨碎补总黄酮(TFRD)联合磷酸钙/胶原支架修复大鼠颅骨缺损的疗效。
方法:受试者,老鼠,分为四组:对照组,TFRD,脚手架,和TFRD+脚手架。颅骨严重骨缺损,直径5mm,是通过精确钻孔人工诱导的。手术后,每隔2、4和8周,进行显微CT扫描以评估颅骨修复的进展。苏木精-伊红和Masson染色技术用于辨别形态学差异,和免疫组织化学染色用于确定局部成骨活性因子的表达水平,如骨形态发生蛋白2(BMP-2)和骨钙蛋白(OCN)。
结果:在8周检查时,支架和TFRD+支架组的颅骨缺损表现出显著的修复,后一组只显示微不足道的foramina。Micro-CT检查相对于其同行公布,TFRD+支架组在4周和8周的间隔内表现出明显的骨再生。值得注意的是,在整个观察期间,与TFRD和支架组相比,TFRD支架组表现出大量的骨缺损修复,而组织形态学评估显示,2周后胶原纤维含量明显高于其他组。免疫组织化学分析进一步证实,相对于其他组,TFRD+支架在2周、4周时BMP-2和2周时OCN的表达增加。
结论:TFRD和磷酸钙/胶原支架的协同应用已被证明可以增强骨矿化,骨可塑性,和骨组织形态学,尤其是在成骨初期。
OBJECTIVE: To investigate the therapeutic efficacy of total flavonoids of Rhizoma Drynariae (TFRD) in conjunction with a calcium phosphate/collagen scaffold for the repair of cranial defects in rats.
METHODS: The subjects, rats, were segregated into four groups: Control, TFRD, Scaffold, and TFRD + Scaffold. Cranial critical bone defects, 5 mm in diameter, were artificially induced through precise drilling. Post-surgery, at intervals of 2, 4, and 8 weeks, micro-CT scans were conducted to evaluate the progress of skull repair. Hematoxylin-eosin and Masson staining techniques were applied to discern morphological disparities, and immunohistochemical staining was utilized to ascertain the expression levels of local osteogenic active factors, such as bone morphogenetic protein 2 (BMP-2) and osteocalcin (OCN).
RESULTS: Upon examination at the 8-week mark, cranial defects in the Scaffold and TFRD + Scaffold cohorts manifested significant repair, with the latter group displaying only negligible foramina. Micro-CT examination unveiled relative to its counterparts, and the TFRD + Scaffold groups exhibited marked bone regeneration at the 4- and 8-week intervals. Notably, the TFRD + Scaffold group exhibited substantial bone defect repair compared to the TFRD and Scaffold groups throughout the entire observation period, while histomorphological assessment demonstrated a significantly higher collagen fiber content than the other groups after 2 weeks. Immunohistochemical analysis further substantiated that the TFRD + Scaffold had augmented expression of BMP-2 at 2, 4 weeks and OCN at 2 weeks relative to other groups.
CONCLUSIONS: The synergistic application of TFRD and calcium phosphate/collagen scaffold has been shown to enhance bone mineralization, bone plasticity, and bone histomorphology especially during initial osteogenesis phases.