Abstract：
Osteoporosis is a metabolic condition distinguished by the degradation of bone microstructure and mechanical characteristics. Traditional Chinese medicine (TCM) has been employed in China for the treatment of various illnesses. Naringin, an ingredient found in Drynariae TCM, is known to have a significant impact on bone metabolism. For this research, we studied the precise potential effect of Drynaria Naringin on protecting against bone loss caused by stress deficiency. In this study, a tail-suspension (TS) test was performed to establish a mouse model with hind leg bone loss. Some mice received subcutaneous injections of Drynaria Naringin for 30 d. Trabecular bone microarchitecture was evaluated using micro-computed tomography analysis and bone histological analysis. Bone formation and resorption markers were quantified in blood samples from mice or in the supernatant of MC3T3-E1 cells by ELISA analysis, Western blotting, and PCR. Immunofluorescence was utilized to visualize the location of β-catenin. Additionally, siRNA was employed to knockdown-specific genes in the cells. Our findings highlight the efficacy of Drynaria Naringin in protecting against the deterioration of bone loss and promoting bone formation and Rspo1 expression in a mouse model following the TS test. Specifically, in vitro experiments also indicated that Drynaria Naringin may promote osteogenesis through the Wnt/β-catenin signalling pathway. Moreover, our results suggest that Drynaria Naringin upregulates the expression of Rspo1/Lgr4, leading to the promotion of osteogenesis via the Wnt/β-catenin signalling pathway. Therefore, Drynaria Naringin holds potential as a therapeutic medication for osteoporosis. Drynaria Naringin alleviates bone loss deterioration caused by mechanical stress deficiency through the Rspo1/Lgr4-mediated Wnt/β-catenin signalling pathway.
摘要：
骨质疏松症是一种代谢状况，其特征在于骨骼微观结构和机械特性的降解。传统中药（TCM）已在中国用于治疗各种疾病。柚林宁,在骨碎补中药中发现的一种成分，已知对骨代谢有显著影响。对于这项研究，我们研究了DrynariaNaringin对防止压力不足引起的骨丢失的确切潜在作用。在这项研究中,进行尾悬吊(TS)试验以建立具有后腿骨丢失的小鼠模型。一些小鼠皮下注射骨碎补柚皮苷30d。使用显微计算机断层扫描分析和骨组织分析评估骨小梁骨微结构。通过ELISA分析对小鼠血液样品或MC3T3-E1细胞上清液中的骨形成和吸收标志物进行定量。西方印迹,和PCR。免疫荧光用于显示β-连环蛋白的位置。此外,使用siRNA敲低细胞中的特异性基因。我们的发现强调了在TS测试后的小鼠模型中，DrynariaNaringin在防止骨质流失恶化，促进骨形成和Rspo1表达方面的功效。具体来说,体外实验还表明骨碎补柚皮苷可能通过Wnt/β-catenin信号通路促进成骨。此外，我们的研究结果表明，乳乳糜泻可上调Rspo1/Lgr4的表达，从而通过Wnt/β-catenin信号通路促进成骨。因此,DrynariaNaringin具有作为骨质疏松症治疗药物的潜力。骨碎补柚皮苷通过Rspo1/Lgr4介导的Wnt/β-catenin信号通路减轻机械应力缺乏引起的骨丢失恶化。
