豌豆(Pisumsativum)品种对豌豆象鼻虫害虫(Bruchuspisorum)的抵抗力很小,强调需要在Pisum种质中寻找抗性来源并破译抗性的遗传基础。为了满足这一需求,我们在Pisum种质资源收集中筛选了对豌豆象鼻虫的反应(324号,先前的基因分型)在四种环境下的田间条件下。确定了象鼻虫种子侵染(SI)的显着变化,富勒菌中经常有抗药性,其次是P.sativumssp。elatius,深渊疟原虫,和P.sativumssp。幽默.种子颜色较浅的种质中SI往往较高。SI也受到环境因素的影响,在开花期间受到高湿度的青睐,在开花期间和之后受到温暖的冬季温度和高蒸散量的阻碍。合并表型和基因型数据可以进行全基因组关联研究(GWAS),得出与SI显着相关的73个标记。通过GWAS模型,发现23个候选基因与象鼻虫抗性相关,突出了位于6号染色体上的五个基因的兴趣。这些包括编码角鲨烯环氧酶的基因127136761;编码转录因子MYBSRM1的基因127091639;编码60S核糖体蛋白L14的基因127097033;编码BolA样家族蛋白的基因127092211,which,有趣的是,位于QTLBpLD内。I,先前描述为赋予豌豆对象鼻虫的抗性;和编码甲基转移酶的基因127096593。这些相关基因为开发抗Bruchusspp的豌豆品种提供了宝贵的潜力。并通过标记辅助选择(MAS)有效利用基因组资源。
Little resistance to the pea weevil insect pest (Bruchus pisorum) is available in pea (
Pisum sativum) cultivars, highlighting the need to search for sources of resistance in
Pisum germplasm and to decipher the genetic basis of resistance. To address this need, we screened the response to pea weevil in a
Pisum germplasm collection (324 accession, previously genotyped) under field conditions over four environments. Significant variation for weevil seed infestation (SI) was identified, with resistance being frequent in P. fulvum, followed by P. sativum ssp. elatius, P. abyssinicum, and P. sativum ssp. humile. SI tended to be higher in accessions with lighter seed color. SI was also affected by environmental factors, being favored by high humidity during flowering and hampered by warm winter temperatures and high evapotranspiration during and after flowering. Merging the phenotypic and genotypic data allowed genome-wide association studies (GWAS) yielding 73 markers significantly associated with SI. Through the GWAS models, 23 candidate genes were found associated with weevil resistance, highlighting the interest of five genes located on chromosome 6. These included gene 127136761 encoding squalene epoxidase; gene 127091639 encoding a transcription factor MYB SRM1; gene 127097033 encoding a 60S ribosomal protein L14; gene 127092211, encoding a BolA-like family protein, which, interestingly, was located within QTL BpLD.I, earlier described as conferring resistance to weevil in pea; and gene 127096593 encoding a methyltransferase. These associated genes offer valuable potential for developing pea varieties resistant to Bruchus spp. and efficient utilization of genomic resources through marker-assisted selection (MAS).