卵巢组织移植可以恢复生育能力;然而,该技术的成功取决于所使用的移植区域。因此,这项研究旨在评估两个皮下区域对犬卵巢移植的影响,耳廓(Pi)和颈部(Ne),7天和15天。使用打孔装置将通过卵巢输卵管子宫切除术收集的卵巢破碎。新鲜的碎片被固定,和其他立即移植到动物本身的Pi和Ne区域7和15天。恢复的碎片进行组织学评估(形态学,发育和基质密度),picrosirius(胶原纤维),和免疫组织化学(纤维化和细胞增殖)。结果表明,Pi-7的卵泡正常率较低(78%)。对照(90%)和Pi-15(86%),与对照相比,Ne-7(92%)相似,Ne-15(97%)优于对照,具有效力的区域Ne(94%)优于Pi(82%)(P<0.05)。两个区域的基质密度降低与控制,但在15天内相似。来自两个区域的片段显示较高的纤连蛋白标记和I型和较低的III型胶原纤维的沉积(P<0.05)。控制。Ne-7的细胞增殖率高于对照组(P<0.05),Pi-15高于Ne-15(P<0.05)。总之,犬卵巢组织自体移植15天后,耳廓可能比颈部具有更大的潜力。
Ovarian tissue transplantation makes it possible to restore fertility; however, the success of this technique depends on the transplant region used. Therefore, this study aimed to evaluate the effect of two subcutaneous regions on canine ovarian transplantation,
pinna (Pi) and neck (Ne), for 7 and 15 days. Ovaries collected by ovariosalpingohysterectomy were fragmented using a punch device. Fresh fragments were fixed, and the others were immediately grafted onto the animal itself in the Pi and Ne regions for 7 and 15 days. Recovered fragments were evaluated for histology (morphology, development and stromal density), picrosirius (collagen fibers), and immunohistochemistry (fibrosis and cell proliferation). The results showed that follicular normality rates were lower in Pi-7 (78%) vs. control (90%) and Pi-15 (86%), similar in Ne-7 (92%) and superior in Ne-15 (97%) compared to the control, with the effect of the region Ne (94%) superior (P < 0.05) to Pi (82%). Stromal density reduced in both regions vs. control but was similar within 15 days. Fragments from both regions showed higher fibronectin labeling and deposition of type I and lower type III collagen fibers (P < 0.05) vs. control. Proliferation rates in Ne-7 were higher (P < 0.05) than in control, and Pi-15 was higher (P < 0.05) than Ne-15. In conclusion, the
pinna may be a region with greater potential than the neck after a 15-day autotransplantation of canine ovarian tissue.