尽管褪黑激素(MT)和钛纳米颗粒(TiNPs)最近已被广泛用于调节植物中的镉(Cd)胁迫,这些材料对降低鼠尾草植物中Cd毒性的比较影响存在差距。这项研究的目的是确定MT和TiNPs的叶面施用如何影响生长,Cd积累,光合作用,含水量,脂质过氧化,以及Cd污染土壤中鼠尾草植物的精油(EO)质量和数量。局部使用100和200μM的MT和50和100mgL-1的TiNPs进行阶乘实验,以及在10和20mgCdkg-1土壤中的Cd毒性。结果表明,Cd毒性降低了植物的生长,增强了脂质过氧化。在20mgkg-1土壤中的Cd胁迫导致Cd根增加(693%),Cd芽(429%),电解液泄漏(EL,29%),丙二醛(MDA,72%),射击重量(31%),根重(27%),叶绿素(Chl)a+b(26%),相对含水量(RWC,23%),和EO产率(30%)。应用MT和TiNPs通过降低MDA和EL来控制干旱胁迫,增加植物重量,Chl,RWC,和环氧乙烷生产,并将植物组织中的Cd积累降至最低。EO中的主要化合物是α-thujone,1,8-桉树脑,β-thujone,樟脑,和α-腐植烯。MT和TiNP导致α-thujone上升,而Cd胁迫导致其下降。基于热图分析,MDA是对处理最敏感的性状。总之,这项研究强调了MT和TiNP的可能性,特别是200μM的MT,以减轻鼠尾草植物中的Cd毒性并增强其生化反应。
Notwithstanding the fact that melatonin (MT) and titanium nanoparticles (Ti NPs) alone have been widely used recently to modulate cadmium (Cd) stress in plants, there is a gap in the comparative impacts of these materials on lowering Cd toxicity in sage plants. The objective of this study was to determine how foliar application of MT and Ti NPs affected the growth, Cd accumulation, photosynthesis, water content, lipid peroxidation, and essential oil (EO) quality and quantity of sage plants in Cd-contaminated soils. A factorial experiment was conducted using MT at 100 and 200 μM and Ti NPs at 50 and 100 mg L-1 topically, together with Cd toxicity at 10 and 20 mg Cd kg-1 soil. The results showed that Cd toxicity decreased plant growth and enhanced lipid peroxidation. The Cd stress at 20 mg kg-1 soil resulted in increases in Cd root (693%), Cd shoot (429%), electrolyte leakage (EL, 29%), malondialdehyde (MDA, 72%), shoot weight (31%), root weight (27%), chlorophyll (Chl) a + b (26%), relative water content (RWC, 23%), and EO yield (30%). The application of MT and Ti NPs controlled drought stress by reducing MDA and EL, enhancing plant weight, Chl, RWC, and EO production, and minimizing Cd accumulation in plant tissues. The predominant compounds in the EO were α-thujone, 1,8-cineole, β-thujone, camphor, and α-humulene. MT and Ti NPs caused α-thujone to rise, whereas Cd stress caused it to fall. Based on heat map analysis, MDA was the trait that was most sensitive to treatments. In summary, the research emphasizes the possibility of MT and Ti NPs, particularly MT at 200 μM, to mitigate Cd toxicity in sage plants and enhance their biochemical reactions.